Fredrikson S, Karlsson-Parra A, Olsson T, Link H
Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
Clin Exp Immunol. 1987 May;68(2):298-304.
HLA-DR expression on T lymphocytes in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and acute aseptic meningo-encephalitis (AM), and from blood only from healthy controls was examined by a new double-immunofluorescence labelling assay using species-specific second layers on prefixed cell samples. Thirteen of 16 patients with AM (81%) had an elevated percentage of DR positive T cells in CSF against only two of 20 patients with MS (10%). Our data indicate that AM, an acute infection of the central nervous system (CNS), is accompanied by accumulation in CSF of activated, DR positive T cells as a reflection of actively involved cellular immunity within the CNS, while this accumulation of DR positive T cells is not seen in MS, a chronic inflammatory CNS disease, despite some of the patients being examined during clinical exacerbations.
采用一种新的双重免疫荧光标记检测法,利用预先固定细胞样本上的种属特异性第二层抗体,检测了多发性硬化症(MS)患者、急性无菌性脑膜脑炎(AM)患者脑脊液(CSF)中以及仅来自健康对照者血液中T淋巴细胞上的HLA - DR表达。16例AM患者中有13例(81%)脑脊液中DR阳性T细胞百分比升高,而20例MS患者中只有2例(10%)出现这种情况。我们的数据表明,AM作为一种中枢神经系统(CNS)的急性感染,其脑脊液中伴有活化的DR阳性T细胞的积聚,这反映了CNS内活跃的细胞免疫参与,而在MS这种慢性炎症性CNS疾病中,尽管部分患者是在临床病情加重期间接受检查,但并未见到DR阳性T细胞的这种积聚。