Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of () against ATCC 25175: A Comparative Study.

OBJECTIVE

To determine the antibacterial effect of four concentrations of the hydroalcoholic extract of "" (HET) against ATCC 25175.

METHODS

This was a prospective, experimental, comparative study. Fermented was subjected to hydric stress to obtain a hydroalcoholic extract at four different concentrations: 100%, 50%, 75%, and 25%. strains were cultured in brain heart infusion agar using the swab technique. The antibacterial effectiveness of HET was evaluated following the Kirby-Bauer disk diffusion method and compared with 0.12% chlorhexidine (positive control group).

RESULTS

The highest mean inhibitory effect was achieved with HET at 100% (33.1 ± 2.2 mm, showing a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)). Inferential analysis found statistically significant differences among all the experimental groups (=0.001). The post hoc analysis also showed significant differences among all the experimental groups evaluated; however, there were no significant differences between HET 50% and chlorhexidine 0.12% ( > 0.05).

CONCLUSIONS

It was found that the highest antibacterial effectiveness was obtained by HET 100%, being even higher than the 0.12% chlorhexidine positive control, and was statistically significant. Post hoc analysis showed that almost all the concentrations showed optimal efficacy against