Kumari Swati, Sharma Anita, Chaudhary Parul, Khati Priyanka
Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145 India.
Crop Production Division, Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263601 India.
3 Biotech. 2020 Nov;10(11):461. doi: 10.1007/s13205-020-02448-2. Epub 2020 Oct 3.
Application of nanocompounds and plant growth promoting rhizobacteria (PGPR) plays an important role in improving plant growth and soil health. In the present study, response of two PGPR (PS2-KX650178 and PS10-KX650179) along with nanozeolite and nanochitosan was studied on Fenugreek (), on the basis of physiological and biochemical parameters of soil and plant in pot experiment for 45 days. A significant increase (1.5-2 folds) in plant height, leaf number, leaf area and fresh weight over control was observed in Fenugreek plants when treated with nanocompounds and PGPR. Combined treatment also showed the highest level of total chlorophyll (3.27 mg g), sugar (6.14 μg mg dry wt), soluble leaf protein (295.37 mg g fresh weight) and catalase activity (23.84 U g tissue) in Fenugreek plants. GC-MS analysis of plant metabolites revealed the abundance of phenols which are known to improve biotic/abiotic stresses in plants. Activity of Fluorescein Diacetate hydrolase enzyme was 2.5 times higher in the combined treatment of nanozeolite with PS10 than in control. An increase of 11% in alkaline phosphatase activity was observed in the same treatment with respect to control. The results obtained from the pot experiment clearly indicate that nanocompounds along with PGPR improved the growth of plants and soil health which suggest their benefits in agriculture practices to increase crop production.
纳米化合物和植物促生根际细菌(PGPR)的应用在改善植物生长和土壤健康方面发挥着重要作用。在本研究中,基于盆栽试验45天内土壤和植物的生理生化参数,研究了两种PGPR(PS2-KX650178和PS10-KX650179)以及纳米沸石和纳米壳聚糖对胡芦巴的影响。用纳米化合物和PGPR处理的胡芦巴植株,其株高、叶片数量、叶面积和鲜重比对照有显著增加(1.5至2倍)。联合处理还使胡芦巴植株中的总叶绿素(3.27毫克/克)、糖分(6.14微克/毫克干重)、可溶性叶片蛋白(295.37毫克/克鲜重)和过氧化氢酶活性(23.84单位/克组织)达到最高水平。对植物代谢物的GC-MS分析显示,已知能改善植物生物/非生物胁迫的酚类物质含量丰富。纳米沸石与PS10联合处理中荧光素二乙酸酯水解酶的活性比对照高2.5倍。在相同处理中,碱性磷酸酶活性相对于对照增加了11%。盆栽试验的结果清楚地表明,纳米化合物与PGPR一起改善了植物生长和土壤健康,这表明它们在农业生产实践中有助于提高作物产量。