淋巴细胞功能相关抗原3（LFA - 3）的一级结构。T淋巴细胞CD2糖蛋白的配体。
Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein.
作者信息
Wallner B P, Frey A Z, Tizard R, Mattaliano R J, Hession C, Sanders M E, Dustin M L, Springer T A
机构信息
Biogen Research Corporation, Cambridge, Massachusetts 02142.
出版信息
J Exp Med. 1987 Oct 1;166(4):923-32. doi: 10.1084/jem.166.4.923.
We have isolated the cDNA for human lymphocyte function-associated antigen 3 (LFA-3), the ligand of the T lymphocyte CD2 molecule. The identity of the clones was established by comparison of the deduced amino acid sequence to the LFA-3 NH2-terminal and tryptic peptide sequences. The cDNA defines a mature protein of 222 amino acids that structurally resembles typical membrane-anchored proteins. An extracellular domain with six N-linked glycosylation sites is followed by a hydrophobic putative transmembrane region and a short cytoplasmic domain. The mature glycoprotein is estimated to be 44-68% carbohydrate. Southern blots of human genomic DNA indicate that only one gene codes for human LFA-3. Northern blot analysis demonstrates that the LFA-3 mRNA of 1.3 kb is widely distributed in human tissues and cell lines.
我们分离出了人类淋巴细胞功能相关抗原3（LFA - 3）的cDNA，它是T淋巴细胞CD2分子的配体。通过将推导的氨基酸序列与LFA - 3的氨基末端和胰蛋白酶肽序列进行比较，确定了克隆的身份。该cDNA定义了一个由222个氨基酸组成的成熟蛋白，其结构类似于典型的膜锚定蛋白。一个具有六个N - 连接糖基化位点的细胞外结构域，接着是一个疏水的假定跨膜区域和一个短的细胞质结构域。估计成熟糖蛋白的碳水化合物含量为44% - 68%。人类基因组DNA的Southern印迹表明，只有一个基因编码人类LFA - 3。Northern印迹分析表明，1.3kb的LFA - 3 mRNA广泛分布于人类组织和细胞系中。
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