从鼠迷走感觉神经节制备用于转录组研究的单细胞悬液的方案。

Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies.

机构信息

Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.

Department of Molecular Neurosciences, Center for Brain Research, Medical University Vienna, 1090 Vienna, Austria.

出版信息

STAR Protoc. 2020 Jun 3;1(1):100030. doi: 10.1016/j.xpro.2020.100030. eCollection 2020 Jun 19.

DOI:10.1016/j.xpro.2020.100030

PMID:33111081

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7580114/

Abstract

Vagal sensory neurons relay viscero- and somatosensory information from within the body and play a key role in maintaining physiological homeostasis. We recently characterized the diversity of vagal sensory neurons in the mouse using a single-cell transcriptomics approach. Here, we provide an in-depth protocol for the extraction of mouse vagal ganglia and the production of high-quality single-cell suspensions from this tissue. This effective protocol can also be applied for use with other peripheral and central neuron populations with few modifications. For complete details on the use and execution of this protocol, please refer to Kupari et al. (2019).

摘要

迷走感觉神经元传递来自体内的内脏和躯体感觉信息，并在维持生理稳态方面发挥着关键作用。我们最近采用单细胞转录组学方法对小鼠的迷走感觉神经元的多样性进行了研究。在这里，我们提供了一个从组织中提取小鼠迷走神经节并制备高质量单细胞悬液的详细方案。此有效方案稍加修改也可应用于其他外周和中枢神经元群体。如需详细了解本方案的使用和执行，请参考 Kupari 等人 (2019)。