用于检测血管生成素-1活性及体内成像的红色发射比率荧光探针。
Red emission ratio fluorescent probe for the activity of vanin-1 and imaging in vivo.
作者信息
Qian Jing, Zhang Liang, Wang Jiemin, Teng Zhidong, Cao Ting, Zheng Lei, Cao Yuping, Qin Wenwu, Liu Yun, Guo Huichen
机构信息
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Key Laboratory of Special Function Materials and Structure Design (MOE), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China.
出版信息
J Hazard Mater. 2021 Jan 5;401:123863. doi: 10.1016/j.jhazmat.2020.123863. Epub 2020 Sep 2.
Pantetheinase, also known as Vanin-1, catalyzes pantetheine to decompose into the precursor of CoA - pantothenic acid and aminothiol cysteamine. Studies have shown that Vanin-1 plays an important role in many important physiological pathologies. In this paper, a new red emission ratio fluorescent probe DCM-PA (I/I) has been implemented to detect the activity of Vanin-1 in cells and vivo. DCM-PA has short response time (30 min), high selectivity and low sensitivity (DL =0.69 ng/mL). Also, we have applied DCM-PA for imaging in cells and mice, and the results have indicated that the probe has a non-negligible potential for monitoring the activity of Vanin-1 in situ, benefiting further to study the role of Vanin-1 in physiology and pathology. In addition, the up-regulation of this enzyme by starvation confirmed the inevitable connection between diabetes and abnormal expression of Vanin-1.
泛硫乙胺酶，也称为Vanin-1，催化泛硫乙胺分解为辅酶A的前体——泛酸和氨基硫醇半胱胺。研究表明，Vanin-1在许多重要的生理病理过程中发挥着重要作用。在本文中，一种新型的红色发射比率荧光探针DCM-PA（I/I）已被用于检测细胞和体内Vanin-1的活性。DCM-PA响应时间短（30分钟），选择性高且灵敏度低（检测限=0.69 ng/mL）。此外，我们已将DCM-PA应用于细胞和小鼠成像，结果表明该探针在原位监测Vanin-1活性方面具有不可忽视的潜力，这有助于进一步研究Vanin-1在生理和病理中的作用。此外，饥饿导致该酶上调，证实了糖尿病与Vanin-1异常表达之间存在必然联系。
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