用于监测和生物成像细胞硒代半胱氨酸的 FRET-ICT 双调制比率荧光传感器。
A FRET-ICT Dual-Modulated Ratiometric Fluorescence Sensor for Monitoring and Bio-Imaging of Cellular Selenocysteine.
机构信息
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, Hunan University of Science and Engineering, Yongzhou 425199, China.
出版信息
Molecules. 2020 Oct 28;25(21):4999. doi: 10.3390/molecules25214999.
Since the fluctuation of cellular selenocysteine (Sec) concentration plays an all-important role in the development of numerous human disorders, the real-time fluorescence detection of Sec in living systems has attracted plenty of interest during the past decade. In order to obtain a faster and more sensitive small organic molecule fluorescence sensor for the Sec detection, a new ratiometric fluorescence sensor was designed based on the fluorescence resonance energy transfer (FRET) strategy with coumarin fluorophore as energy donor and 4-hydroxy naphthalimide fluorophore (with 2,4-dinitrobenzene sulfonate as fluorescence signal quencher and Sec-selective recognition site) as an energy acceptor. The sensor exhibited only a blue fluorescence signal, and displayed two well distinguished emission bands (blue and green) in the presence of Sec with ∆λ of 68 nm. Moreover, concentrations ranging of quantitative detection of Sec of was from 0 to 45 μM (limit of detection = 6.9 nM), with rapid ratiometric response, high sensitivity and selectivity capability. Impressively, the results of the living cell imaging test demonstrated has the potentiality of being an ideal sensor for real-time Sec detection in biosystems.
由于细胞硒代半胱氨酸（Sec）浓度的波动在许多人类疾病的发展中起着至关重要的作用，因此在过去十年中，实时荧光检测活系统中的 Sec 引起了广泛关注。为了获得更快、更灵敏的用于 Sec 检测的小分子有机荧光传感器，我们基于荧光共振能量转移（FRET）策略，以香豆素荧光团作为能量供体，以 4-羟基萘二甲酰亚胺荧光团（带有 2,4-二硝基苯磺酸盐作为荧光信号猝灭剂和 Sec 选择性识别位点）作为能量受体，设计了一种新型的比率荧光传感器 。该传感器 仅显示蓝色荧光信号，并且在存在 Sec 时显示两个明显区分的发射带（蓝色和绿色），具有 68nm 的 ∆λ。此外，Sec 的定量检测浓度范围为 0 至 45 μM（检测限= 6.9 nM），具有快速的比率响应、高灵敏度和选择性。令人印象深刻的是，活细胞成像测试的结果表明 具有成为生物系统中实时 Sec 检测的理想传感器的潜力。
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