Shandong University, Jinan 250012, Shandong, China; Department of Ophthalmology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China.
Department of Ophthalmology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China.
J Chem Neuroanat. 2020 Dec;110:101876. doi: 10.1016/j.jchemneu.2020.101876. Epub 2020 Oct 29.
The death of retinal ganglion cells (RGCs) during acute glaucoma causes progressive degeneration of the retinal nerve and irreversible blindness. Astaxanthin (AST) is a type of xanthophyll carotenoids and naturally synthesized by multiple halobios. It has been reported to protect the retina from acute glaucoma due to its anti-oxidative and anti-neuroinflammatory properties. However, the mechanism underlying this process remains unclear. We designed a mouse model with acute glaucoma and AST was administered by oral gavage. Hematoxylin and eosin staining was utilized to evaluate the condition of retina and the number of ganglion cells was counted. QRT-PCR was performed to evaluate the mRNA levels of Bax and Bcl2 while Western blot assay was used to determine the protein levels of Bax, Bcl2, Nrf2 and HO-1. AST protected the retinal integrity of mice with acute glaucoma. The apoptosis of RGCs induced by ischemia and reperfusion was repressed by AST. The protective functions of AST on the retinal and ganglion cells decreased with the knock-down of Nrf2. AST promoted the activation of Nrf2 and Ho-1 in the RGCs of the model mice. AST protected the RGCs from apoptosis during acute glaucoma and alleviated the severe retinopathy symptoms through the Nrf2/Ho-1 pathway.
视网膜神经节细胞 (RGCs) 在急性青光眼期间的死亡导致视网膜神经进行性退化和不可逆转的失明。虾青素 (AST) 是一种叶黄素类胡萝卜素，由多种卤生物自然合成。由于其抗氧化和抗炎神经特性，它已被报道可保护视网膜免受急性青光眼的侵害。然而，这一过程的机制尚不清楚。我们设计了一种急性青光眼小鼠模型，并通过口服灌胃给予 AST。苏木精和伊红染色用于评估视网膜状况并计算神经节细胞数量。实时定量 PCR 用于评估 Bax 和 Bcl2 的 mRNA 水平，Western blot 用于测定 Bax、Bcl2、Nrf2 和 HO-1 的蛋白水平。AST 保护了急性青光眼小鼠的视网膜完整性。AST 抑制了缺血再灌注诱导的 RGCs 凋亡。Nrf2 敲低后，AST 对视网膜和神经节细胞的保护作用降低。AST 促进了模型小鼠 RGCs 中 Nrf2 和 Ho-1 的激活。AST 通过 Nrf2/Ho-1 通路保护急性青光眼期间的 RGCs 免受凋亡，并缓解严重的视网膜病变症状。
Zotero 插件