所选香料对盐酸利多卡因牙科注射液的细胞毒性及掩味效果评估

Evaluation of Cytotoxicity and Taste-Masking Effect of Selected Flavors on Dental Lidocaine HCl Injection.

作者信息

Boddu Sai H S, Tukaramrao Diwakar B, Al-Tabakha Moawia M, Ashames Akram, Neupane Rabin, Babu R Jayachandra, Renukuntla Jwala, Tiwari Amit K

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Ajman University, P.O. Box 346, Ajman, UAE.

Department of Pharmacology and Experimental Therapeutics, The University of Toledo, Health Science Campus, 3000 Arlington Ave, Toledo, OH 43614, USA.

出版信息

Pharmaceuticals (Basel). 2020 Oct 29;13(11):353. doi: 10.3390/ph13110353.

DOI:10.3390/ph13110353

PMID:33138130

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7693877/

Abstract

AIM

Anxiety and intolerance to dental local anesthetic injections are common in patients undergoing dental procedures. This work was designed to study cytotoxicity of selected flavors in primary gingival keratinocytes (PGK), to acquire information on their suitability for use in dental lidocaine hydrochloride (LID) injection. We also evaluated the bio-mimetic taste of LID dental injection in the presence of selected flavors and sweetener using an Astree electronic tongue (ETongue).

METHODS

The cytotoxicity of chocolate natural and artificial flavor (CTE), raspberry flavor artificial (RAS), cherry flavor (CHR), bitterness suppressor flavor (BSF) and lemon flavor extract (LFE) at various dilutions (0.16-10% /) was carried out in PGK using the live cell morphological analysis and MTT cell cytotoxicity assay. Based on the cytotoxicity data, CTE and RAS were added to Xylocaine (2%) along with 0.09% sodium saccharin and taste was assessed using an ETongue.

RESULTS

After three hours of treatment, a dose-dependent cell death was induced by all flavors compared to the untreated control. BSF was found to be more toxic when compared to other flavors. CTE was found to be less toxic. The mean IC50 values of CTE, RAS, CHR, BSF and LFE in PGK were found to be 9.54, 8.43, 2.21, 0.38 and 4.01 mg/mL. Taste analysis with the ETongue showed a clear taste difference between the control and test formulations containing CTE and RAS flavors along with sodium saccharin.

CONCLUSION

CTE and RAS flavors in combination with 0.09% sodium saccharin can achieve a significant taste-masking effect in the dental LID injection.

摘要

目的

在接受牙科治疗的患者中，焦虑和对牙科局部麻醉注射不耐受的情况很常见。本研究旨在研究所选调味剂对原代牙龈角质形成细胞（PGK）的细胞毒性，以获取其用于牙科盐酸利多卡因（LID）注射的适用性信息。我们还使用Astree电子舌（ETongue）评估了在存在所选调味剂和甜味剂的情况下LID牙科注射剂的仿生味道。

方法

使用活细胞形态分析和MTT细胞毒性试验，在PGK中对不同稀释度（0.16 - 10% /）的巧克力天然和人工调味剂（CTE）、树莓人工调味剂（RAS）、樱桃调味剂（CHR）、苦味抑制剂调味剂（BSF）和柠檬调味提取物（LFE）进行细胞毒性试验。根据细胞毒性数据，将CTE和RAS添加到赛罗卡因（2%）中，并加入0.09%的糖精钠，然后使用ETongue评估味道。

结果

与未处理的对照相比，处理三小时后，所有调味剂均诱导了剂量依赖性细胞死亡。发现BSF比其他调味剂毒性更大。发现CTE毒性较小。PGK中CTE、RAS、CHR、BSF和LFE的平均IC50值分别为9.54、8.43、2.21、0.38和4.01 mg/mL。用ETongue进行的味道分析表明，含有CTE和RAS调味剂以及糖精钠的对照配方和测试配方之间存在明显的味道差异。