对T细胞缺陷小鼠实验性念珠菌病的研究。

Studies of experimental candidiasis in T-cell-deficient mice.

作者信息

Sinha B K, Prasad S, Monga D P

机构信息

Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):203-9. doi: 10.1016/s0176-6724(87)80167-0.

DOI:10.1016/s0176-6724(87)80167-0

PMID:3314257

Abstract

The role of cell-mediated immunity (CMI) in the protection against experimental murine candidiasis was studied. CMI-deficient animals prepared by adult thymectomy and treatment with antimouse thymocyte serum along with controls were challenged with one LD50 (6.6 X 10(4)) dose of Candida albicans. The course of infection in these animals was studied by mortality pattern, delayed type hypersensitivity reaction (DTH) to candida antigen, antibody formation, and viable count of organisms in kidney, spleen and liver at different time intervals. The T-cell-deficient animals were found to be highly susceptible to infection with C. albicans. A correlation was observed in the appearance of DTH and protection.

摘要

研究了细胞介导免疫（CMI）在抵抗实验性小鼠念珠菌病中的作用。通过成年胸腺切除和用抗小鼠胸腺细胞血清处理制备的CMI缺陷动物以及对照组，用一个半数致死剂量（LD50，6.6×10⁴）的白色念珠菌进行攻击。通过死亡率模式、对念珠菌抗原的迟发型超敏反应（DTH）、抗体形成以及在不同时间间隔对肾脏、脾脏和肝脏中生物体的活菌计数，研究了这些动物的感染过程。发现T细胞缺陷动物对白色念珠菌感染高度易感。观察到DTH的出现与保护作用之间存在相关性。