MSC-MCF-7 双重体系在鸡胚尿囊膜上诱导肿瘤血管生成和血管发生。
The MSC-MCF-7 Duet Playing Tumor Vasculogenesis and Angiogenesis onto the Chick Embryo Chorioallantoic Membrane.
机构信息
Department of Microscopic Morphology/Histology, "Victor Babeş" University of Medicine and Pharmacy, Timişoara, Romania.
Angiogenesis Research Center, "Victor Babeş" University of Medicine and Pharmacy, Timişoara, Romania.
出版信息
In Vivo. 2020 Nov-Dec;34(6):3315-3325. doi: 10.21873/invivo.12170.
BACKGROUND/AIM: Human mesenchymal stem cells (hMSC) represent a versatile cell population, able to modulate the tumor microenvironment. Our aim was to recreate an open scene for the in vivo interaction between hMSC and the MCF-7 breast cancer cells (MCF-7), in order to enlighten the intimate involvement of hMSC in tumor vasculogenesis and angiogenesis.
MATERIALS AND METHODS
hMSC and MCF-7 were seeded onto the chick embryo chorioallantoic membrane (CAM) and incubated for 7 days. Consecutively, the morphology and the immunohistochemical profile of CAM were assessed.
RESULTS
Following this complex interaction, MCF-7 acquired a more aggressive phenotype, hMSC switched to a vascular precursor phenotype, while CAM underwent a major reset to an earlier stage, with hotspots of angiogenesis, vasculogenesis and hematopoiesis.
CONCLUSION
The hallmark of this study was the establishment of a veritable in vivo experimental model of MSC involvement in tumor vasculogenesis and angiogenesis, allowing further analysis in the field.
背景/目的：人骨髓间充质干细胞（hMSC）是一种多功能细胞群体，能够调节肿瘤微环境。我们的目的是在体内重现 hMSC 与 MCF-7 乳腺癌细胞（MCF-7）相互作用的开放场景，以阐明 hMSC 与肿瘤血管生成和血管生成的密切关系。
材料和方法
将 hMSC 和 MCF-7 接种到鸡胚绒毛尿囊膜（CAM）上，并孵育 7 天。然后，评估 CAM 的形态和免疫组织化学特征。
结果
经过这种复杂的相互作用，MCF-7 获得了更具侵袭性的表型，hMSC 转变为血管前体细胞表型，而 CAM 经历了一个重大的重置，进入一个更早的阶段，出现了血管生成、血管生成和造血的热点。
结论
这项研究的特点是建立了一个真正的 MSC 参与肿瘤血管生成和血管生成的体内实验模型，为该领域的进一步分析提供了可能。
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