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新世界螺旋蝇对有机磷杀虫剂抗性的分子基础。

Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly.

机构信息

Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo (USP), São Paulo, SP, Brazil.

Bayer Crop Science, Rua Domingos Jorge, 1100, São Paulo, SP, Brasil.

出版信息

Parasit Vectors. 2020 Nov 10;13(1):562. doi: 10.1186/s13071-020-04433-3.

DOI:10.1186/s13071-020-04433-3
PMID:33168079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7653728/
Abstract

BACKGROUND

The emergence of insecticide resistance is a fast-paced example of the evolutionary process of natural selection. In this study, we investigated the molecular basis of resistance in the myiasis-causing fly Cochliomyia hominivorax (Diptera: Calliphoridae) to dimethyl-organophosphate (OP) insecticides.

METHODS

By sequencing the RNA from surviving larvae treated with dimethyl-OP (resistant condition) and non-treated larvae (control condition), we identified genes displaying condition-specific polymorphisms, as well as those differentially expressed.

RESULTS

Both analyses revealed that resistant individuals have altered expression and allele-specific expression of genes involved in proteolysis (specifically serine-endopeptidase), olfactory perception and cuticle metabolism, among others. We also confirmed that resistant individuals carry almost invariably the Trp251Ser mutation in the esterase E3, known to confer OP and Pyrethroid resistance. Interestingly, genes involved in metabolic and detoxifying processes (notably cytochrome P450s) were found under-expressed in resistant individuals. An exception to this were esterases, which were found up-regulated.

CONCLUSIONS

These observations suggest that reduced penetration and aversion to dimethyl-OP contaminated food may be important complementary strategies of resistant individuals. The specific genes and processes found are an important starting point for future functional studies. Their role in insecticide resistance merits consideration to better the current pest management strategies.

摘要

背景

杀虫剂抗性的出现是自然选择进化过程的一个快速例子。在这项研究中，我们研究了致蝇蛆的苍蝇 Cochliomyia hominivorax（双翅目：Calliphoridae）对二甲基有机磷（OP）杀虫剂产生抗性的分子基础。

方法

通过对用二甲基-OP（抗性条件）处理的存活幼虫和未处理幼虫（对照条件）的 RNA 进行测序，我们鉴定了显示条件特异性多态性的基因，以及那些差异表达的基因。

结果

这两种分析都表明，抗性个体中涉及蛋白水解（特别是丝氨酸内肽酶）、嗅觉感知和表皮代谢等的基因表达和等位基因特异性表达发生了改变。我们还证实，抗性个体几乎总是携带酯酶 E3 中的 Trp251Ser 突变，该突变已知可赋予 OP 和拟除虫菊酯抗性。有趣的是，在抗性个体中发现参与代谢和解毒过程（特别是细胞色素 P450s）的基因表达下调。酯酶是一个例外，发现其上调。

结论

这些观察结果表明，减少二甲基-OP 污染食物的穿透和厌恶可能是抗性个体的重要补充策略。发现的特定基因和过程是未来功能研究的重要起点。它们在杀虫剂抗性中的作用值得考虑，以改善当前的害虫管理策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/5935ee83b80d/13071_2020_4433_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/3d75f95a23c6/13071_2020_4433_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/772e8b5c59e1/13071_2020_4433_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/5935ee83b80d/13071_2020_4433_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/3d75f95a23c6/13071_2020_4433_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/772e8b5c59e1/13071_2020_4433_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0789/7653728/5935ee83b80d/13071_2020_4433_Fig3_HTML.jpg

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2
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Nat Commun. 2018 Dec 11;9(1):5282. doi: 10.1038/s41467-018-07615-x.
3
Insect cuticle: a critical determinant of insecticide resistance.
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Electrophoresis. 2025 Jan;46(1-2):112-126. doi: 10.1002/elps.202400171. Epub 2025 Jan 9.
4
Morphological and Molecular Characterizations of (Diptera: Calliphoridae) Larvae Responsible for Wound Myiasis in French Guiana.法属圭亚那致伤口蝇蛆病的丽蝇科（双翅目）幼虫的形态学和分子特征
Diagnostics (Basel). 2023 Aug 2;13(15):2575. doi: 10.3390/diagnostics13152575.
5
A chromosomal-scale reference genome of the New World Screwworm, Cochliomyia hominivorax.新世界螺旋蝇 Cochliomyia hominivorax 的染色体级参考基因组。
DNA Res. 2023 Feb 1;30(1). doi: 10.1093/dnares/dsac042.
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4
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6
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8
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9
Cutaneous Myiasis.皮肤蝇蛆病
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10
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Sci Rep. 2016 Apr 20;6:24707. doi: 10.1038/srep24707.