sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste.

A novel e-waste-degrading strain, PE08, was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08 was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 °C (range, 5-40 °C), with 1-2 % NaCl (range, 0-3 %) and at pH 7 (range 6-11). The phylogeny based on 16S rRNA gene sequences delineated strain PE08 to the genus and showed highest sequence similarity to KF707 (98.70 %), followed by DSM 50071 (98.62 %) and DSM 21078 (97.93 %). The genome of strain PE08 was sequenced and had one scaffold of 6056953 bp, 99.84 % completeness and 182× coverage were obtained. The G+C content in the genome was 64.24 mol%. The DNA-DNA hybridization and average nucleotide identity values between strain PE08 and its closely related type strain, DSM 21078 were below 34.8 % and 87.96 %, respectively. The phylogenetic analysis based on whole-genome sequence and concatenated GyrB and RpoB proteins revealed that strain PE08 forms a district clade in the family . The predominant fatty acids were summed feature 8 (Cω7 and/or C ω6), summed feature 3 (Cω7 and/or Cω6), C and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The phenotypic, chemotaxonomic and genetic analysis, including overall genome relatedness index values, indicated that strain PE08 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PE08 (=MCC 3792=KCTC 72454=CCUG 73691).