原生生物细胞与曼氏血吸虫孢子的相互作用。
Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts.
机构信息
Fundação Oswaldo Cruz, Instituto René Rachou, Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias, Diretoria de Pesquisa e Desenvolvimento, Belo Horizonte, MG, Brasil.
出版信息
Rev Soc Bras Med Trop. 2020 Nov 6;53:e20200257. doi: 10.1590/0037-8682-0257-2020. eCollection 2020.
INTRODUCTION
Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria.
METHODS
The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts.
RESULTS
S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described.
CONCLUSIONS
Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
简介
豆螺可能对曼氏血吸虫感染表现出不同程度的易感性。我们一直在开发一种体外模型来研究蜗牛和寄生虫之间的相互作用，使用源自比氏豆螺的组织衍生细胞培养物。
方法
从抗性（比氏豆螺 Taim）和易感（比氏豆螺 HM 和光滑豆螺 BH）比氏豆螺原代培养的消化腺和肾脏衍生细胞中，暴露于曼氏血吸虫孢囊蚴。
结果
曼氏血吸虫孢囊蚴仅被比氏豆螺 Taim 的消化腺（DG）衍生细胞包围和包裹。随后，培养基中游离孢囊蚴的数量明显减少。描述了培养中 DG 衍生细胞的形态特征。
结论
比氏豆螺 Taim 的 DG（而非 SK）原代培养物衍生的细胞可能参与曼氏血吸虫孢囊蚴的控制。
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