Ventura-Martinez Rosa, Angeles-Lopez Guadalupe Esther, Gonzalez-Trujano Maria Eva, Carrasco Omar F, Deciga-Campos Myrna
Departamento de Farmacologia, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Av Universidad No. 3000, Col. Copilco Universidad, Ciudad Universitaria, 04510 Ciudad de México, Mexico.
Laboratorio de Neurofarmacologia de Productos Naturales, Departamento de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría "Ramón de La Fuente Muñiz", México-Xochimilco 101, Col. San Lorenzo Huipulco, 14370 Ciudad de México, Mexico.
Evid Based Complement Alternat Med. 2020 Oct 28;2020:7140642. doi: 10.1155/2020/7140642. eCollection 2020.
has been used in traditional medicine as a remedy to alleviate several gastrointestinal disorders that provoke stomachaches, abdominal cramps, and diarrhea. However, there is not enough scientific evidence that supports these effects. Therefore, the purpose of this study was to evaluate antispasmodic and antidiarrheal activities of aqueous extract of (AqExt-TL) as well as its mechanism of action in experimental models. Antispasmodic activity and the mechanism of action of AqExt-TL were assessed on segments of the guinea pig ileum precontracted with KCl, acetylcholine (ACh), or electrical field stimulation (EFS). Furthermore, the antispasmodic effect of two coumarins (umbelliferone and herniarin) previously identified in this species was evaluated. Antidiarrheal activity of AqExt-TL was determined using the charcoal meal test in mice. AqExt-TL showed antispasmodic activity in segments of the guinea pig ileum precontracted with KCl (83.7 ± 1.9%) and ACh (77.2 ± 5.3%) at the maximal concentration; however, practically, it did not alter the contractions induced by EFS (10.1 ± 2.2%). Antispasmodic activity of AqExt-TL was not significantly altered by hexamethonium (a ganglionic blocker) or L-NAME (an inhibitor of nitric oxide synthase). However, this extract decreased the maximal contractile response to calcium (82.7 ± 8.5%), serotonin (68.1 ± 8.5%), and histamine (63.9 ± 5.9%) in their concentration-response curves. Umbelliferone and herniarin also induced an antispasmodic effect on tissues precontracted with KCl. In addition, low doses of AqExt-TL reduced to 50% the distance traveled by charcoal meal in the gastrointestinal transit model in mice as loperamide, an antidiarrheal agent, did. These results provided evidence of the antispasmodic and antidiarrheal activity of , which supports its use in the folk medicine in relieving symptoms in some gastrointestinal disorders. In the antispasmodic effect, the blockade of histaminergic and serotoninergic pathway as well as the calcium channels seems to be involved. Finally, umbelliferone and herniarin could be partially responsible for the antispasmodic activity induced by .
在传统医学中，它被用作缓解多种胃肠道疾病的药物，这些疾病会引发胃痛、腹部绞痛和腹泻。然而，目前尚无足够的科学证据支持这些功效。因此，本研究的目的是评估[提取物名称]水提取物（AqExt-TL）的解痉和止泻活性及其在实验模型中的作用机制。在氯化钾（KCl）、乙酰胆碱（ACh）或电场刺激（EFS）预收缩的豚鼠回肠段上评估AqExt-TL的解痉活性及其作用机制。此外，还评估了先前在该物种中鉴定出的两种香豆素（伞形花内酯和蛇床子素）的解痉作用。使用小鼠炭末试验测定AqExt-TL的止泻活性。AqExt-TL在最大浓度下对KCl（83.7±1.9%）和ACh（77.2±5.3%）预收缩的豚鼠回肠段显示出解痉活性；然而，实际上，它并未改变EFS诱导的收缩（10.1±2.2%）。六甲铵（一种神经节阻滞剂）或L-精氨酸甲酯（一氧化氮合酶抑制剂）对AqExt-TL的解痉活性没有显著影响。然而，该提取物在其浓度-反应曲线中降低了对钙（82.7±8.5%）、5-羟色胺（68.1±8.5%）和组胺（63.9±5.9%）的最大收缩反应。伞形花内酯和蛇床子素对KCl预收缩的组织也有解痉作用。此外，低剂量的AqExt-TL使小鼠胃肠道转运模型中炭末的移动距离减少了50%，与止泻药洛哌丁胺的效果相同。这些结果提供了[提取物名称]解痉和止泻活性的证据，支持其在民间医学中用于缓解某些胃肠道疾病症状。在解痉作用中，似乎涉及组胺能和5-羟色胺能途径以及钙通道的阻断。最后，伞形花内酯和蛇床子素可能部分负责[提取物名称]诱导的解痉活性。
