Medical Physiology Department, Faculty of Medicine, Zagazig University, Al-Sharquia, Egypt.
Human Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, Al-Sharquia, Egypt.
Can J Physiol Pharmacol. 2021 Jul;99(7):708-719. doi: 10.1139/cjpp-2020-0499. Epub 2020 Nov 17.
-acetylcysteine (NAC) and melatonin were reported to exert protective effects on testicular tissues. Thus, this study aimed to determine which of these is more efficient against obesity-induced testicular dysfunction in albino rats. A total of 32 adult male rats (195 ± 10 g) were divided into four groups: control, obese rats fed a high-fat diet (HFD), HFD+NAC (150 mg/kg per day, i.p.) and HFD+melatonin (10 mg/kg per day, i.p.), for 5 weeks. Testes and epididymis were weighed. Lipid profile, pituitary-testicular hormones, tumor necrosis factor α (TNFα), epididymal sperm parameters, testicular oxidant-antioxidant system, testicular and the epididymal histopathology and immunohistochemical localization for androgen receptors (AR) and Bax reaction were analyzed. Administration of NAC or melatonin significantly improved the lipid parameters, gonadal hormones, TNFα level, sperm count and abnormal morphology, oxidant-antioxidant system and the absolute testicular and epididymal mass with an enhancement of testicular architecture, AR expression and apoptosis as compared with that in the obese group. Additionally, as compared with the NAC group, the melatonin group had significantly reduced body mass index, total cholesterol, triglyceride, and TNFα and increased testosterone, sperm count, motility, superoxide dismutase activity, mitigated histomorphometrical changes, Bax expression, and increased testicular AR expression. Therefore, melatonin was more efficient than NAC in affording fortification against HFD-induced testicular dysfunction.
-乙酰半胱氨酸（NAC）和褪黑素据称对睾丸组织具有保护作用。因此，本研究旨在确定这两种物质中哪一种对肥胖诱导的白化大鼠睾丸功能障碍更有效。总共 32 只成年雄性大鼠（195±10g）被分为四组：对照组、高脂饮食喂养的肥胖大鼠（HFD）、HFD+NAC（150mg/kg/天，腹腔注射）和 HFD+褪黑素（10mg/kg/天，腹腔注射），共 5 周。测量睾丸和附睾的重量。分析血脂谱、垂体-睾丸激素、肿瘤坏死因子 α（TNFα）、附睾精子参数、睾丸氧化应激-抗氧化系统、睾丸和附睾组织病理学以及雄激素受体（AR）和 Bax 反应的免疫组织化学定位。与肥胖组相比，给予 NAC 或褪黑素可显著改善血脂参数、性腺激素、TNFα 水平、精子计数和异常形态、氧化应激-抗氧化系统以及绝对睾丸和附睾质量，同时增强睾丸结构、AR 表达和细胞凋亡。此外，与 NAC 组相比，褪黑素组的体重指数、总胆固醇、甘油三酯和 TNFα 显著降低，而睾酮、精子计数、活力、超氧化物歧化酶活性增加，组织形态学变化减轻，Bax 表达减少，睾丸 AR 表达增加。因此，褪黑素在预防 HFD 诱导的睾丸功能障碍方面比 NAC 更有效。
