在没有金标准检测方法的情况下，验证一种多重 PCR 检测方法，以检测乌拉圭牛中的 spp. 和 。
Validation of a multiplex PCR assay to detect spp. and in cattle in Uruguay in the absence of a gold standard test.
机构信息
Instituto Nacional de Investigación Agropecuaria, Plataforma de Salud Animal, Estación Experimental INIA, Tacuarembó, Uruguay.
Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
J Vet Diagn Invest. 2021 Jan;33(1):73-79. doi: 10.1177/1040638720975742. Epub 2020 Nov 30.
Detection of bovine spp. and is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine spp. and by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the gene region of and , and the region of . We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of spp. were 68.6% and 99.1%, and for 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for spp. were 94.2% and 97.1%, and for 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting spp. and , and would be a reliable test for veterinarians to choose the correct treatment for each agent.
牛巴贝斯虫和无形体的检测基于吉姆萨染色的血液或器官涂片的读取，其敏感性可能较低。我们的目的是通过验证多重 PCR（mPCR）来提高牛巴贝斯虫和无形体的检测率。我们使用了 466 份有巴贝斯虫病或无形体病临床症状和疑似剖检发现的动物的血液和/或器官样本。我们的 mPCR 引物扩增了 和 的 基因区域，以及 的 区域。我们使用具有非信息先验分布的贝叶斯模型来估计流行率，以及具有信息先验分布的敏感性和特异性估计。涂片检测牛巴贝斯虫的敏感性和特异性分别为 68.6%和 99.1%，检测无形体的敏感性和特异性分别为 85.6%和 98.8%。mPCR 检测牛巴贝斯虫的敏感性和特异性分别为 94.2%和 97.1%，检测无形体的敏感性和特异性分别为 95.2%和 92.7%。我们的 mPCR 在检测牛巴贝斯虫和无形体方面具有良好的准确性，并且将成为兽医选择针对每种病原体进行正确治疗的可靠检测方法。
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