Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer.

PURPOSE

The long noncoding RNA 1 () contributes to hepatocellular carcinoma development. However, its expression and roles in colorectal cancer (CRC) have not been fully explored. Therefore, this study determined the expression and roles of in CRC and elucidated its detailed mechanism of action.

METHODS

expression in CRC tissues and cell lines was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We used Cell Counting Kit-8, flow cytometry, cell migration and invasion assays, and a xenograft tumor model to test the effects of on CRC malignancy. The associations among , microRNA-484 (miR-484), and () were explored using luciferase reporter assay, RNA immunoprecipitation, RT-qPCR, and Western blotting.

RESULTS

was overexpressed in CRC and related to poor prognosis. interference inhibited CRC cell proliferation, migration, and invasion but induced apoptosis. Furthermore, deficiency impaired tumor growth in vivo. Mechanistically, adsorbed miR-484, thereby increasing the expression of its target . Rescue experiments revealed that the effects of deficiency in CRC cells were reversed by inhibiting miR-484 or upregulating .

CONCLUSION

drives CRC progression by sponging miR-484 and consequently upregulating . The /miR-484/ pathway may serve as a diagnostic and therapeutic target for CRC.