Tonisi Sipho, Okaiyeto Kunle, Mabinya Leonard V, Okoh Anthony I
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa.
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa.
Saudi J Biol Sci. 2020 Dec;27(12):3559-3569. doi: 10.1016/j.sjbs.2020.07.028. Epub 2020 Aug 1.
() is a bulbous tropical and subtropical flowering plant widespread in Africa, which is frequently used to treat several human ailments. Until the present, there is no scientific validation on the biological activity of this plant from the Eastern Cape Province of South Africa and as a result, this study aimed to assess the bioactive compounds, free radicals scavenging and anticancer potentials of crude bulb extracts (chloroform, acetone, and ethanol) of obtained from this geographical location. Standard biochemical techniques and Gas-chromatography mass spectrometry (GCMS) analysis were used to pinpoint the bioactive compounds in the crude extracts sequel to their antioxidant potentials against radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide and nitric oxide as well as their ferric ion reducing power. In addition, their cytotoxicity effects against Human cervix adenocarcinoma (HeLa) cells were assessed as an in vitro model for anticancer. The phytochemical evaluation of the crude extracts showed the presence of phenolics, flavonoids, and alkaloids. GCMS profiles confirmed the presence of some bioactive compounds in the crude extracts of that could be responsible for their biological activities. The plant extracts possessed considerable antioxidant activity and exhibited dose-dependent radicals' inhibition from all assays carried out. Furthermore, the cytotoxicity effects against HeLa cells recorded inhibition concentration (IC) of 1.5, 1.6, and 1.9 µg/mL for acetone, chloroform, and ethanolic extracts of , respectively. Findings from the present study suggest that could be a good prospective source of antioxidant and anticancer agents. Therefore, further research on the isolation and purification of compounds from these extracts are indispensable.
（）是一种球根状的热带和亚热带开花植物，在非洲广泛分布，常用于治疗多种人类疾病。直到目前，对于这种来自南非东开普省的植物的生物活性尚无科学验证，因此，本研究旨在评估从该地理位置获得的球根粗提物（氯仿、丙酮和乙醇提取物）的生物活性化合物、自由基清除能力和抗癌潜力。采用标准生化技术和气相色谱 - 质谱联用（GCMS）分析来确定粗提物中的生物活性化合物，随后测定其对2,2 - 二苯基 - 1 - 苦基肼（DPPH）、2,2'-联氮 - 双 -（3 - 乙基苯并噻唑啉 - 6 - 磺酸）（ABTS）、过氧化氢和一氧化氮等自由基的抗氧化潜力以及它们的铁离子还原能力。此外，评估了它们对人宫颈腺癌（HeLa）细胞的细胞毒性作用，作为抗癌的体外模型。粗提物的植物化学评估表明存在酚类、黄酮类和生物碱。GCMS图谱证实了该植物粗提物中存在一些可能与其生物活性相关的生物活性化合物。植物提取物具有相当的抗氧化活性，并且在所有进行的测定中均表现出剂量依赖性的自由基抑制作用。此外，对HeLa细胞的细胞毒性作用记录显示，该植物的丙酮、氯仿和乙醇提取物的抑制浓度（IC）分别为1.5、1.6和1.9 μg/mL。本研究结果表明该植物可能是抗氧化剂和抗癌剂的良好潜在来源。因此，对从这些提取物中分离和纯化化合物进行进一步研究是必不可少的。
