琉璃苣油可增加必需脂肪酸缺乏豚鼠板层小体内容物并改变表皮神经酰胺的脂肪酸组成。

Borage Oil Enhances Lamellar Body Content and Alters Fatty Acid Composition of Epidermal Ceramides in Essential Fatty Acid-Deficient Guinea Pigs.

机构信息

Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, 1732, Deogyeong-daero, Yongin-si, 17104, Republic of Korea.

Department of Food Science and Nutrition, Hallym University, 1, Hallymdaehak-gil, Chuncheon-si, 24252, Republic of Korea.

出版信息

Lipids. 2021 May;56(3):345-353. doi: 10.1002/lipd.12295. Epub 2020 Dec 30.

DOI:10.1002/lipd.12295

PMID:33378788

Abstract

Borage oil [BO: 40.9% linoleic acid (LNA) and 24.0% γ-linolenic acid (GLA)] reverses disrupted epidermal lipid barrier in essential fatty acid deficiency (EFAD). We determined the effects of BO on lamellar body (LB) content and LNA and GLA incorporation into epidermal ceramide 1 (CER1) and epidermal ceramide 2 (CER2), major barrier lipids. EFAD was induced in guinea pigs by a diet of 6% hydrogenated coconut oil (HCO) for 10 weeks (group HCO) or 8 weeks followed by 6% BO for 2 weeks (group HCO + BO). LB content and LNA and GLA incorporation into CER1 were higher in group HCO + BO than in group HCO. Small but significant levels of LNA, GLA, and their C20-metabolized fatty acids [dihomo-γ-linolenic acid (DGLA) and arachidonic acid (ARA)] were incorporated into CER2, where ARA was detected at a level lower than LNA, but DGLA incorporation exceeded that for GLA in group HCO + BO. Dietary BO enhanced LB content and differential incorporation of GLA into CER1 and DGLA into CER2.

摘要

琉璃苣油[40.9%亚油酸（LNA）和 24.0%γ-亚麻酸（GLA）]可逆转必需脂肪酸缺乏症（EFAD）中表皮脂质屏障的破坏。我们确定了 BO 对板层小体（LB）含量以及 LNA 和 GLA 掺入表皮神经酰胺 1（CER1）和表皮神经酰胺 2（CER2）的影响，这是主要的屏障脂质。通过用 6%氢化椰子油（HCO）喂养 10 周（HCO 组）或 8 周后用 6%BO 喂养 2 周（HCO+BO 组）在豚鼠中诱导 EFAD。HCO+BO 组的 LB 含量以及 LNA 和 GLA 掺入 CER1 的水平均高于 HCO 组。LNA、GLA 及其 C20 代谢脂肪酸[二高-γ-亚麻酸（DGLA）和花生四烯酸（ARA）]以小但显着的水平掺入 CER2，其中 ARA 的水平低于 LNA，但 DGLA 的掺入量超过了 HCO+BO 组中的 GLA。膳食 BO 增强了 LB 含量以及 GLA 对 CER1 的差异掺入和 DGLA 对 CER2 的掺入。