基于透明质酸的三维微环境可有效诱导心血管祖细胞群体。
Hyaluronan-Based Three-Dimensional Microenvironment Potently Induces Cardiovascular Progenitor Cell Populations.
作者信息
Gluck Jessica M, Chyu Jennifer, Delman Connor, Heydarkhan-Hagvall Sepideh, MacLellan W Robb, Shemin Richard J
机构信息
Cardiovascular Tissue Engineering Laboratory, Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, 10833 Le Conte Avenue, 62-151 CHS, Los Angeles, CA 90095-1741, USA.
Cardiovascular Research Laboratory, Departments of Medicine and Cardiology, David Geffen School of Medicine, University of California, Los Angeles, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA 90095, USA.
出版信息
ISRN Tissue Eng. 2013;2013. doi: 10.1155/2013/752620. Epub 2013 Sep 3.
The relationship between stem cell niches and their surrounding microenvironment is still relatively unknown. Recent advances have indicated that extrinsic factors within the cardiovascular progenitor cell niche influence maintenance of a multipotent state as well as drive cell-fate decisions. We have previously shown the direct effects of extracellular matrix (ECM) proteins and have now investigated the effects of dimension on the induction of a cardiovascular progenitor cell (CPC) population. We have shown here that the three-dimensionality of a hyaluronan-based hydrogel greatly induces a CPC population, as marked by Flk-1. We have compared the effects of a 3D microenvironment to those of conventional 2D cell culture practices and have found that the 3D microenvironment potently induces a progenitor cell state.
干细胞生态位与其周围微环境之间的关系仍相对未知。最近的进展表明，心血管祖细胞生态位内的外在因素会影响多能状态的维持以及驱动细胞命运的决定。我们之前已经展示了细胞外基质（ECM）蛋白的直接作用，现在又研究了维度对心血管祖细胞（CPC）群体诱导的影响。我们在此表明，基于透明质酸的水凝胶的三维结构极大地诱导了CPC群体，以Flk-1为标志。我们将三维微环境的作用与传统二维细胞培养方法的作用进行了比较，发现三维微环境能有效诱导祖细胞状态。
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