碘化褪黑素模拟褪黑素作用并揭示胎儿脑中的离散结合位点。

Iodinated melatonin mimics melatonin action and reveals discrete binding sites in fetal brain.

作者信息

Weaver D R, Namboodiri M A, Reppert S M

机构信息

Laboratory of Developmental Chronobiology, Children's Service, Massachusetts General Hospital, Boston 02114.

出版信息

FEBS Lett. 1988 Feb 8;228(1):123-7. doi: 10.1016/0014-5793(88)80599-4.

DOI:10.1016/0014-5793(88)80599-4

PMID:3342869

Abstract

Iodinated melatonin was used to study melatonin sites of action in brain. Iodomelatonin mimicked the effects of melatonin on reproductive development in Djungarian hamster fetuses. 125I-melatonin injected into the dam was recovered from fetal brain. In vitro autoradiographic studies revealed a remarkably discrete distribution of competitive 125I-melatonin-binding sites in the fetal brain, with binding in median eminence/arcuate nucleus area greater than suprachiasmatic nucleus greater than pineal gland much greater than anterior pituitary gland much greater than preoptic area. 125I-melatonin promises to be a useful tool for understanding the sites and mechanism of action of melatonin.

摘要

碘化褪黑素被用于研究褪黑素在大脑中的作用位点。碘代褪黑素模拟了褪黑素对黑线仓鼠胎儿生殖发育的影响。注入母体的125I-褪黑素可在胎儿大脑中检测到。体外放射自显影研究显示，胎儿大脑中竞争性125I-褪黑素结合位点分布极为离散，在正中隆起/弓状核区域的结合大于视交叉上核大于松果体远大于垂体前叶远大于视前区。125I-褪黑素有望成为了解褪黑素作用位点和作用机制的有用工具。