Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, 160-8582, Japan.
Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, 160-8582, Japan.
Virology. 2021 Mar;555:35-43. doi: 10.1016/j.virol.2020.12.020. Epub 2021 Jan 6.
The pandemic of COVID-19 is still ongoing, and many studies on serum antibodies have been reported, however, there are few studies about asymptomatic and mild patients. In this study, we enrolled 44 COVID-19 patients with relatively mild disease and 48 pre-pandemic controls. We measured serum antibodies against extracellular domain, S1 domain, and receptor-binding domain of Spike and N protein, examined neutralization titers by authentic virus neutralization assay and newly-developed bead/cell-based Spike-ACE2 inhibition assay, and compared them with clinical features. Most of these antibodies, including neutralizing titers, were mutually correlated, and the production of antibodies were associated with low Ct values of PCR test, disease severity, symptoms especially pneumonia, lymphopenia, and serological test including CRP, LD, D-dimer, and procalcitonin. Notably, 87.5% of asymptomatic and 23.5% of mild patients did not have antibody against SARS-CoV-2. Our results revealed the inadequate acquisition of humoral immunity in patients with asymptomatic and mild COVID-19 patients.
COVID-19 大流行仍在持续，已有许多关于血清抗体的研究报告，但针对无症状和轻症患者的研究较少。在这项研究中，我们招募了 44 名 COVID-19 轻症患者和 48 名大流行前的对照者。我们测量了针对 Spike 和 N 蛋白胞外域、S1 域和受体结合域的血清抗体，通过真实病毒中和测定和新开发的基于珠粒/细胞的 Spike-ACE2 抑制测定来检测中和效价，并将其与临床特征进行比较。这些抗体中的大多数，包括中和效价，都是相互关联的，抗体的产生与 PCR 检测的低 Ct 值、疾病严重程度、症状（尤其是肺炎）、淋巴细胞减少以及包括 CRP、LD、D-二聚体和降钙素原在内的血清学检测有关。值得注意的是，87.5%的无症状患者和 23.5%的轻症患者没有针对 SARS-CoV-2 的抗体。我们的结果揭示了无症状和轻症 COVID-19 患者中体液免疫的获得不足。
