Department of Entomology and Plant Pathology, University of Arkansas Systems Division of Agriculture, Fayetteville, AR, USA.
Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
Fungal Genet Biol. 2021 Mar;148:103519. doi: 10.1016/j.fgb.2021.103519. Epub 2021 Jan 17.
Cytoplasmic dynein is a minus end-directed microtubule motor that can be activated by cargo adapters. In Aspergillus nidulans, overexpression of ΔC-HookA, the early endosomal adapter HookA missing its cargo-binding site, causes activated dynein to accumulate at septa and spindle pole bodies (SPBs) where the microtubule-organizing centers are located. Intriguingly, only some interphase nuclei show SPB signals of dynein. Here we present data demonstrating that localization of the activated dynein at SPBs is cell cycle-dependent: SPB dynein signals are seen to associate with nuclei at early G1 but disappear at about the G1-S boundary.
细胞质动力蛋白是一种向微管负端定向的微管运动蛋白，可以被货物衔接蛋白激活。在构巢曲霉中，过表达早期内体衔接蛋白 HookA 的 ΔC-HookA 结构（缺失其货物结合位点），导致激活的动力蛋白在隔膜和纺锤体极体（微管组织中心所在的位置）处积累。有趣的是，只有一些间核显示动力蛋白的 SPB 信号。在这里，我们提供的数据表明，激活的动力蛋白在 SPB 上的定位是细胞周期依赖性的：在早期 G1 时观察到 SPB 动力蛋白信号与核结合，但在大约 G1-S 边界处消失。
