研究具有良好抗氧化活性的新型桑椹球状多糖。
Study on a novel spherical polysaccharide from Fructus Mori with good antioxidant activity.
机构信息
School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; SCUT-Zhuhai Institute of Modern Industrial Innovation, Zhuhai, 519715, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China.
出版信息
Carbohydr Polym. 2021 Mar 15;256:117516. doi: 10.1016/j.carbpol.2020.117516. Epub 2020 Dec 16.
A novel polysaccharide (MFP1P) was isolated from Fructus Mori, followed by purification via DEAE-52 cellulose and 27 % ethanol fraction. The MFP1P had the molecular weight of 56.78 kDa and the total sugar content of 93.32±0.54 %. And the MFP1P is mainly composed of glucose, galactose, galacturonic acid and mannose with molar ratio of 66.62 %, 13.94 %, 18.24 % and 1.20 %, respectively. MFP1P was mainly composed of →3)-α-D-Gal (1→, β-D-Man-(1→ and →6)-α-D-Glc (1→ glycosidic bond and showed a spherical chain conformation with uniform distribution in solution. The MFP1P exhibited great antioxidant activity with oxygen-free radical absorption capacity (ORAC) values of 291.63±6.81 μmol TE/g and MDA IC of 0.289±0.022 mg/mL.
从桑椹中分离得到一种新型多糖（MFP1P），并通过 DEAE-52 纤维素和 27%乙醇分级进行纯化。MFP1P 的分子量为 56.78 kDa，总糖含量为 93.32±0.54%。MFP1P 主要由葡萄糖、半乳糖、半乳糖醛酸和甘露糖组成，摩尔比分别为 66.62%、13.94%、18.24%和 1.20%。MFP1P 主要由→3）-α-D-Gal（1→、β-D-Man-（1→和→6）-α-D-Glc（1→糖苷键组成，在溶液中呈球形链构象，分布均匀。MFP1P 具有很强的抗氧化活性，其氧自由基吸收能力（ORAC）值为 291.63±6.81 μmol TE/g，MDA IC 值为 0.289±0.022 mg/mL。
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