Padhan Bhagyashree, Poddar Kasturi, Sarkar Debapriya, Sarkar Angana
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha, 769008, India.
Biotechnol Rep (Amst). 2021 Jan 16;29:e00592. doi: 10.1016/j.btre.2021.e00592. eCollection 2021 Mar.
A pink pigment-producing bacterial strain was isolated from wastewater and identified as sp. BPW19. The motile bacterial strain was Gram-positive, acid fermenting, glucose, sucrose utilizing and rod-shaped with an average cell length of 1.55 μm as studied under the Environmental Scanning Electron Microscope. Even though being psychrotolerant, the cell growth condition of BPW19 was optimized as 25 ºC along with pH 8, and 2.25% inoculum concentration considering the operational ease of the production. Sonication assisted solvent extraction produced 5.41% crude pigment which showed zones of exclusion against gram-negative strains DH5α, sp. EtK3, and sp. SHC1. Gas Chromatography-Mass Spectrometry analysis of the crude pigment exhibited the dominant presence of major compounds as dotriacontane; 3,7 dimethyl 7 octanal; 1-eicosene and erucic acid. While column chromatography (ethanol:chloroform in 1:4 (v/v) ratio) purified pigment was identified as erucic acid using Nuclear Magnetic Resonance with a net yield of 3.06%.
从废水中分离出一株产粉色色素的细菌菌株，鉴定为BPW19菌属。在环境扫描电子显微镜下观察，该运动性细菌菌株为革兰氏阳性，能发酵产酸，利用葡萄糖和蔗糖，呈杆状，平均细胞长度为1.55μm。尽管BPW19具有耐冷性，但考虑到生产操作的简便性，其细胞生长条件优化为25℃、pH值8以及接种浓度2.25%。超声辅助溶剂萃取法得到了5.41%的粗色素，该粗色素对革兰氏阴性菌株DH5α、EtK3菌属和SHC1菌属表现出抑菌圈。对粗色素进行气相色谱-质谱分析，结果显示主要化合物为三十二烷、3,7-二甲基-7-辛醛、1-二十碳烯和芥酸。采用柱色谱法（乙醇:氯仿体积比为1:4）纯化色素，通过核磁共振鉴定为芥酸，净产率为3.06%。
