祛瘀生肌方通过抑制前列腺素转运体的表达促进糖尿病伤口愈合。
Quyu Shengji Formula Facilitates Diabetic Wound Healing via Inhibiting the Expression of Prostaglandin Transporter.
作者信息
Lu Yi, Ding Xiaojie, Qi Fei, Ru Yi, Kuai Le, Chen Siting, Yang Yingyao, Li Xin, Li Fulun, Li Bin, Zhou Mi, Ze Kan
机构信息
Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine/Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China.
Department of Vascular, Shanghai Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China.
出版信息
Evid Based Complement Alternat Med. 2021 Jan 22;2021:8849935. doi: 10.1155/2021/8849935. eCollection 2021.
BACKGROUND
Quyu Shengji Formula (QSF), a Chinese medicine formula widely used in the clinic, has proven therapeutic effects on diabetic ulcers. Nevertheless, the potential mechanism of how QSF cures diabetic ulcer remains elusive.
OBJECTIVE
To assess the mechanism of QSF against wound healing defects in diabetes.
METHODS
Db/db mice were adopted to determine the therapeutic potential of QSF. Further histology analysis was performed by hematoxylin and eosin (H&E) staining. Moreover, the expression patterns of prostaglandin transporter (PGT), prostaglandin E (PGE), and angiogenesis factor vascular endothelial growth factor (VEGF) were evaluated by immunostaining (IHC) analysis, ELISA assay, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot analysis . Human dermal microvascular endothelial cells (HDMECs) and the shRNA interference technique were used to explore the effects of QSF on cell migration, PGT, PGE, and angiogenesis factor VEGF .
RESULTS
Applied QSF on the wound of db/db mice significantly accelerated wound closure. Reductions of PGT and elevations of PGE and increased angiogenesis factor VEGF levels were shown after QSF treatment and Furthermore, QSF promoted HDMEC migration. Inhibition of the expression of PGT by shRNA reversed phenotypes of QSF treatment .
CONCLUSION
Taken together, our findings reveal that QSF ameliorates diabetes-associated wound healing defects by abolishing the expression of PGT.
背景
祛瘀生肌方（QSF）是一种临床广泛应用的中药方剂，已证实对糖尿病溃疡有治疗作用。然而，QSF治疗糖尿病溃疡的潜在机制仍不清楚。
目的
评估QSF改善糖尿病伤口愈合缺陷的机制。
方法
采用db/db小鼠确定QSF的治疗潜力。通过苏木精-伊红（H&E）染色进行进一步的组织学分析。此外，通过免疫染色（IHC）分析、酶联免疫吸附测定（ELISA）、实时定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析评估前列腺素转运体（PGT）、前列腺素E（PGE）和血管生成因子血管内皮生长因子（VEGF）的表达模式。使用人真皮微血管内皮细胞（HDMECs）和短发夹RNA（shRNA）干扰技术探讨QSF对细胞迁移、PGT、PGE和血管生成因子VEGF的影响。
结果
将QSF应用于db/db小鼠伤口可显著加速伤口愈合。QSF治疗后显示PGT降低、PGE升高以及血管生成因子VEGF水平增加。此外，QSF促进HDMEC迁移。shRNA抑制PGT表达可逆转QSF治疗的表型。
结论
综上所述，我们的研究结果表明，QSF通过消除PGT的表达改善糖尿病相关的伤口愈合缺陷。
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