Novel Strategy of Mussel-Inspired Immobilization of Naringinase with High Activity Using a Polyethylenimine/Dopamine Co-deposition Method.

Mussel-inspired surface chemistry is recognized as a simple, efficient, and mild surface modification method and has become a research hotspot in many fields. In this study, polyethylenimine/dopamine was coated on the surface of SBA-15 using a co-deposition method, making it possible to immobilize naringinase with high activity and operation stability. The optimal modification and immobilization conditions as well as enzyme properties were investigated. The naringinase activity can reach up to 753.78 U/g carrier, which was much higher than those of the previous works. Besides, the residual naringinase activity still kept 78.91% of the initial activity after one month of storage and maintained 60.79% after 8 cycles. Therefore, the strategy of mussel-inspired enzyme immobilization could be recognized as a promising and universal enzyme immobilization method, with the advantages of high relative enzyme activity, enzyme carrying rate, enzyme activity recovery rate, and good reusability and storage stability.