School of Life Sciences, Lanzhou University, Lanzhou, 730000, People's Republic of China.
College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830046, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Apr;114(4):399-410. doi: 10.1007/s10482-021-01524-8. Epub 2021 Feb 15.
A Gram-stain negative, aerobic, rod-shaped, motile by a single polar flagellum, non-spore-forming bacterium, designated strain AL-54, was isolated from the storage liquid in the stems of Populus euphratica tree at the ancient Ugan River in Xinjiang, PR China. Isolated AL-54 grew optimally at pH 7.0 and temperature 35 °C in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence demonstrated that the isolate belonged to the genus Pseudomonas and was closely related to Pseudomonas songnenensis NEAU-ST5-5 (97.6%), Pseudomonas zhaodongensis NEAU-ST5-21 (97.5%), Pseudomonas alcaliphila AL15-21 (97.3%), Pseudomonas toyotomiensis HT-3 (97.3%), Pseudomonas oleovorans subsp. lubricantis RS1 (97.3%), Pseudomonas stutzeri ATCC 17588 (97.3%), Pseudomonas chengduensis CGMCC 2318 (97.2%), and Pseudomonas xanthomarina KMM 1447 (97.1%). Multilocus Sequences Analysis (MLSA) of strain AL-54 based on the three housekeeping genes, rpoB, rpoD and gyrB further confirmed the phylogenetic assignment of the isolates. The G+C content was 64.7 mol%. The DNA-DNA hybridization with P. songnenensis NEAU-ST5-5 , P. zhaodongensis NEAU-ST5-21, P. alcaliphila AL15-21, P. toyotomiensis HT-3, P. oleovorans subsp. lubricantis RS1, P. stutzeri ATCC 17588, P. chengduensis CGMCC 2318 and P. xanthomarina KMM 1447 revealed 44.0%, 44.7%, 60.1%, 48.7%, 49.1%, 60.1%, 58.9% and 60.2% relatedness respectively. The predominant quinone system is ubiquinone-9 (Q-9). The major components of the cellular fatty acids (>10%) were summed feature 8 (comprising C ω7c /C ω6c), summed feature 3 (comprising C ω7c /C ω6c) and C. The detected major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylcholine (PC). On the basis of phenotypic data, chemotaxonomic and phylogenetic properties, strain AL-54 can consider as a novel species within the genus Pseudomonas, for which the name Pseudomonas lopnurensis sp. nov. is proposed. The type strain is AL-54 (= JCM 19136 = CCTCC AB 2013066 = NRRL B-59987).
一株革兰氏阴性、需氧、杆状、单极鞭毛运动、不产芽孢的细菌，被命名为 AL-54 菌株，从中国新疆古老的额尔齐斯河胡杨树干的储存液中分离得到。AL-54 菌株在 pH 值为 7.0、温度为 35°C、含有 3%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，该分离株属于假单胞菌属，与假单胞菌属松嫩亚种 NEAU-ST5-5（97.6%）、假单胞菌属肇东亚种 NEAU-ST5-21（97.5%）、假单胞菌属嗜碱亚种 AL15-21（97.3%）、假单胞菌属丰原亚种 HT-3（97.3%）、假单胞菌属奥利沃氏亚种 RS1（97.3%）、假单胞菌属施特劳斯亚种 ATCC 17588（97.3%）、假单胞菌属成都亚种 CGMCC 2318（97.2%）和假单胞菌属黄单胞菌亚种 KMM 1447（97.1%）最为接近。基于 rpoB、rpoD 和 gyrB 三个看家基因的菌株 AL-54 的多位点序列分析（MLSA）进一步证实了分离株的系统发育归属。G+C 含量为 64.7 mol%。与假单胞菌属松嫩亚种 NEAU-ST5-5、假单胞菌属肇东亚种 NEAU-ST5-21、假单胞菌属嗜碱亚种 AL15-21、假单胞菌属丰原亚种 HT-3、假单胞菌属奥利沃氏亚种 RS1、假单胞菌属施特劳斯亚种 ATCC 17588、假单胞菌属成都亚种 CGMCC 2318 和假单胞菌属黄单胞菌亚种 KMM 1447 的 DNA-DNA 杂交显示，相关性分别为 44.0%、44.7%、60.1%、48.7%、49.1%、60.1%、58.9%和 60.2%。主要醌类为泛醌-9（Q-9）。细胞脂肪酸的主要成分（>10%）为特征性 8 组（包括 C ω7c /C ω6c）、特征性 3 组（包括 C ω7c /C ω6c）和 C。检测到的主要极性脂为磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、双磷脂酰甘油（DPG）和磷脂酰胆碱（PC）。基于表型数据、化学生态学和系统发育特性，菌株 AL-54 可被认为是假单胞菌属中的一个新种，命名为洛浦假单胞菌 sp. nov.。其模式菌株为 AL-54（=JCM 19136=CCTCC AB 2013066=NRRL B-59987）。
