Marzejon Marcin J, Kornaszewski Łukasz, Bogusławski Jakub, Ciąćka Piotr, Martynow Miłosz, Palczewska Grażyna, Maćkowski Sebastian, Palczewski Krzysztof, Wojtkowski Maciej, Komar Katarzyna
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warszawa, Poland.
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, G. Narutowicza 11/12, 80-223 Gdańsk, Poland.
Biomed Opt Express. 2020 Dec 17;12(1):462-479. doi: 10.1364/BOE.411168. eCollection 2021 Jan 1.
Two-photon vision is a phenomenon associated with the perception of short pulses of near-infrared radiation (900-1200 nm) as a visible light. It is caused by the nonlinear process of two-photon absorption by visual pigments. Here we present results showing the influence of pulse duration and repetition rate of short pulsed lasers on the visual threshold. We compared two-photon sensitivity maps of the retina obtained for subjects with normal vision using a cost-effective fiber laser ( = 1028.4 nm, = 12.2 ps, = 19.17 MHz) and a solid-state laser ( = 1043.3 nm, = 0.253 ps, = 62.65 MHz). We have shown that in accordance with the description of two-photon absorption, the average optical power required for two-photon vision for a fiber laser is 4 times greater than that for a solid-state laser. Mean sensitivity measured for the first one is 5.9 ± 2.8 dB lower than for the second but still 17 dB away from the safety limit, confirming that picosecond light sources can be successfully applied in microperimetry. This development would dramatically reduce the cost and complexity of future clinical devices.
双光子视觉是一种与将近红外辐射（900 - 1200纳米）的短脉冲感知为可见光相关的现象。它是由视觉色素的双光子吸收这一非线性过程引起的。在此，我们展示了短脉冲激光的脉冲持续时间和重复率对视觉阈值影响的结果。我们比较了使用具有成本效益的光纤激光器（波长 = 1028.4纳米，脉宽 = 12.2皮秒，重复率 = 19.17兆赫兹）和固态激光器（波长 = 1043.3纳米，脉宽 = 0.253皮秒，重复率 = 62.65兆赫兹）为正常视力受试者获得的视网膜双光子敏感度图。我们已经表明，根据双光子吸收的描述，光纤激光器实现双光子视觉所需的平均光功率比固态激光器大4倍。测量得到的前者的平均敏感度比后者低5.9 ± 2.8分贝，但仍比安全极限低17分贝，这证实了皮秒光源可成功应用于微视野计。这一进展将大幅降低未来临床设备的成本和复杂性。
