用于检测大肠杆菌不耐热肠毒素的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
作者信息
Yolken R H, Greenberg H B, Merson M H, Sack R B, Kapikian A Z
出版信息
J Clin Microbiol. 1977 Nov;6(5):439-44. doi: 10.1128/jcm.6.5.439-444.1977.
Abstract
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
摘要
本文描述了一种用于检测不耐热大肠杆菌肠毒素的酶联免疫吸附测定（ELISA）方法的开发。该测定基于霍乱弧菌毒素与不耐热大肠杆菌肠毒素之间的免疫相似性，其设计与放射免疫测定相似，但使用酶标记而非放射性同位素标记的试剂。ELISA系统在检测不耐热大肠杆菌肠毒素方面与放射免疫测定和Y-1肾上腺细胞测定一样灵敏，但既不需要放射性试剂也不需要组织培养技术。ELISA操作简便，适用于小型实验室。
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