Katzenberger Ruth Hanna, Rösel Anja, Vonberg Ralf-Peter
Institute for Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
BMC Res Notes. 2021 Mar 15;14(1):97. doi: 10.1186/s13104-021-05492-0.
Environmental surfaces may serve as potential reservoirs for nosocomial pathogens and facilitate transmissions via contact depending on its tenacity. This study provides data on survival kinetics of the most important nosocomial bacteria on a panel of commonly used surfaces. Type strains of S. aureus, K. pneumoniae, P. aeruginosa, A. baumannii, S. marcescens, E. faecium, E. coli, and E. cloacae were suspended in 0.9% NaCl solution at a McFarland of 1 and got then plated via cotton swabs either on glass, polyvinyl chloride, stainless steel, or aluminum. Surfaces were stored at regular ambient temperature and humidity to simulate routine daycare conditions. Sampling was performed by contact plates for a time period of four weeks.
The longest survival was observed for A. baumannii and E. faecium on all materials (at least four weeks). S. aureus remained viable for at least one week. Gram negative species other than A. baumannii were usually inactivated in less than two days. Nosocomial transmission of the above mentioned bacteria may easily occur if no appropriate infection control measures are applied on a regular daily basis. This might be of particular importance when dealing with outbreaks of A. baumannii and E. faecium.
环境表面可能作为医院病原体的潜在储存库，并根据其持久性通过接触促进传播。本研究提供了一组常用表面上最重要的医院细菌存活动力学数据。金黄色葡萄球菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌、粘质沙雷氏菌、粪肠球菌、大肠杆菌和阴沟肠杆菌的标准菌株悬浮于0.9%氯化钠溶液中，使其麦氏比浊度为1，然后用棉拭子接种于玻璃、聚氯乙烯、不锈钢或铝表面。将表面置于正常的环境温度和湿度下以模拟日常护理条件。通过接触平板进行为期四周的采样。
在所有材料上，鲍曼不动杆菌和粪肠球菌存活时间最长（至少四周）。金黄色葡萄球菌至少存活一周。除鲍曼不动杆菌外的革兰氏阴性菌通常在两天内失活。如果日常不采取适当的感染控制措施，上述细菌的医院内传播可能很容易发生。在应对鲍曼不动杆菌和粪肠球菌暴发时，这可能尤为重要。
