韩国非结核分枝杆菌肺病患者的肺部微生物组。
The lung microbiota in Korean patients with non-tuberculous mycobacterial pulmonary disease.
机构信息
Division of Pulmonology and Allergy, Department of Internal Medicine, Gil Medical Center, Gachon University College of Medicine, 21, Namdong-daero 774 beon-gil, Namdong-gu, Incheon, 21565, Republic of Korea.
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, Republic of Korea.
出版信息
BMC Microbiol. 2021 Mar 18;21(1):84. doi: 10.1186/s12866-021-02141-1.
BACKGROUND
The microbiota of the lower respiratory tract in patients with non-tuberculous mycobacterial pulmonary disease (NTM-PD) has not been fully evaluated. We explored the role of the lung microbiota in NTM-PD by analyzing protected specimen brushing (PSB) and bronchial washing samples from patients with NTM-PD obtained using a flexible bronchoscope.
RESULTS
Bronchial washing and PSB samples from the NTM-PD group tended to have fewer OTUs and lower Chao1 richness values compared with those from the control group. In both bronchial washing and PSB samples, beta diversity was significantly lower in the NTM-PD group than in the control group (P = 2.25E-6 and P = 4.13E-4, respectively). Principal component analysis showed that the PSBs and bronchial washings exhibited similar patterns within each group but differed between the two groups. The volcano plots indicated differences in several phyla and genera between the two groups.
CONCLUSIONS
The lower respiratory tract of patients with NTM-PD has a unique microbiota distribution that is low in richness/diversity.
背景
非结核分枝杆菌肺病（NTM-PD）患者下呼吸道的微生物群尚未得到充分评估。我们通过分析使用柔性支气管镜从 NTM-PD 患者获得的保护性标本刷（PSB）和支气管冲洗样本，探讨了肺部微生物群在 NTM-PD 中的作用。
结果
与对照组相比，NTM-PD 组的支气管冲洗和 PSB 样本的 OTU 数量较少，Chao1 丰富度值较低。在支气管冲洗和 PSB 样本中，NTM-PD 组的 beta 多样性均明显低于对照组（P=2.25E-6 和 P=4.13E-4）。主成分分析表明，每组内的 PSB 和支气管冲洗均表现出相似的模式，但两组之间存在差异。火山图表明两组之间有几个门和属存在差异。
结论
NTM-PD 患者的下呼吸道具有独特的微生物群分布，其丰富度/多样性较低。
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