诱导多能干细胞向间充质干细胞的分化。
Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells.
机构信息
Department of Dermatology, University of Colorado School of Medicine, Aurora, CO, USA.
Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
出版信息
Methods Mol Biol. 2022;2549:153-167. doi: 10.1007/7651_2021_383.
Differentiating human induced pluripotent stem cells (iPSCs) into multipotent mesenchymal stem/stromal cells (MSCs) offers a renewable source of therapeutically invaluable cells. However, the process of MSC derivation from iPSCs suffers from an undesirably low efficiency. In this chapter, we present an optimized procedure to produce MSCs from human iPSCs with a high efficiency. The protocol depends on the generation of embryoid bodies (EBs) and requires the treatment of EBs with transforming growth factor beta 1 (TGF-β1). The resulting MSCs can be purified based on the expression of CD73, CD105, and CD90 markers and expanded for multiple passages without losing their characteristics.
将人诱导多能干细胞（iPSCs）分化为多能间充质干细胞（MSCs）为治疗提供了极具价值的可再生细胞来源。然而，iPSC 向 MSC 分化的过程效率不理想。在本章中，我们提出了一种优化的从人 iPSC 高效生产 MSC 的方法。该方案依赖于类胚体（EBs）的生成，并需要用转化生长因子β 1（TGF-β1）处理 EBs。得到的 MSC 可以根据 CD73、CD105 和 CD90 标志物的表达进行纯化，并可在不丢失其特征的情况下进行多次传代扩增。
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