一种优化的单子叶植物组织 RNA 杂交方案。

Institute for Molecular Physiology, Heinrich-Heine-University Düsseldorf, Düsseldorf 40225, Germany.

Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent 9052, Belgium.

STAR Protoc. 2021 Mar 17;2(2):100398. doi: 10.1016/j.xpro.2021.100398. eCollection 2021 Jun 18.

RNA hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections. For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021).

RNA 杂交可能既耗时又难以解决。在这里，我们提供了一个优化的玉米叶片组织方案，尽管它也可以应用于其他植物组织，如茎尖分生组织、胚胎和花器官。我们为每个感兴趣的基因生成三个>100 bp 的独特反义探针，并将它们与组织切片杂交。有关该方案使用和执行的完整详细信息，请参考 Bezrutczyk 等人（2021 年）。