[穴位埋线远近端对原发性痛经大鼠子宫前列腺素、血清白细胞介素-2及脾自然杀伤细胞活性的影响]

[Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats].

作者信息

Yang Wen-Wen, Chen Pan-Bi, Jin Ling-Min, Tang Xu-Yun, Qin Zhong-Yin, Zhou Yang-Jiaqi, Chen Yi

机构信息

Graduate School of Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China.

College of Acupuncture and Orthopedics, Guizhou University of Traditional Chinese Medicine, Guiyang 550002.

出版信息

Zhen Ci Yan Jiu. 2021 Mar 25;46(3):221-5. doi: 10.13702/j.1000-0607.200618.

DOI:10.13702/j.1000-0607.200618

PMID:33798295

Abstract

OBJECTIVE

To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E (PGE) and prostaglandin F(PGF) in the uterus, serum Interleukin-2 (IL-2) contents and splenic natural killer (NK) cell activity in primary dysmenorrhea (PD) rats，so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats.

METHODS

Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate (0.5 mg/rat on the first day and 10th day, and 0.2 mg/rat from the 2nd to the 9th day) and intraperitoneal injection of oxytocin (2 U/rat, on the 11th day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao" (SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE2 and PGF2αin uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry.

RESULTS

Following modeling, the body writhing times of the model group was increased than that of the control group (<0.01); After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group (<0.01). Compared with the control group, the uterine mass and uterus PGF content were increased (<0.01), while uterus PGE, serum IL-2 and splenic NK cell activity were decreased (<0.01) in the model group. After interventions, the uterine mass and uterus PGF were down-regulated (<0.01, <0.05), while the contents of uterus PGE, serum IL-2 and splenic NK cell activity up-regulated (<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF was down-regulated (<0.05) and splenic NK cell activity was up-regulated (<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group.

CONCLUSION

Both proximal and distal acupoint catgut-embeding can increase PGE and decrease PGF in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding.

摘要

目的

观察原发性痛经（PD）大鼠子宫中前列腺素E（PGE）和前列腺素F（PGF）含量、血清白细胞介素-2（IL-2）含量及脾自然杀伤（NK）细胞活性，探讨远近端穴位埋线对PD大鼠的不同影响。

方法

将雌性Wistar大鼠随机分为对照组、模型组、近端穴位埋线组和远端穴位埋线组，每组8只。采用皮下注射苯甲酸雌二醇（第1天和第10天0.5mg/只，第2至9天0.2mg/只）和腹腔注射缩宫素（第11天2U/只）建立PD模型。近端穴位埋线组于双侧“关元”（CV4）和“次髎”（BL32）埋线，远端穴位埋线组于双侧“三阴交”（SP6）和“地机”（SP8）埋线。治疗后，记录30分钟内的扭体次数和子宫质量，采用ELISA法检测子宫中PGE2和PGF2α以及血清中IL-2的含量，采用MTT比色法检测脾NK细胞活性。

结果

造模后，模型组扭体次数较对照组增加（<0.01）；干预后，近端和远端穴位埋线组扭体次数较模型组减少（<0.01）。与对照组比较，模型组子宫质量和子宫PGF含量增加（<0.01），而子宫PGE、血清IL-2和脾NK细胞活性降低（<0.01）。干预后，与模型组比较，近端和远端穴位埋线组子宫质量和子宫PGF下调（<0.01，<0.05），而子宫PGE、血清IL-2含量及脾NK细胞活性上调（<0.01）。近端穴位埋线组子宫PGF含量下调（<0.05），脾NK细胞活性上调（<0.01），优于远端穴位埋线组。