Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2021 Mar 3;22(5):2512. doi: 10.3390/ijms22052512.
Chloroplasts cannot develop normally without the coordinated action of various proteins and signaling connections between the nucleus and the chloroplast genome. Many questions regarding these processes remain unanswered. Here, we report a novel P-type pentatricopeptide repeat (PPR) factor, named Albino Cotyledon Mutant1 (ACM1), which is encoded by a nuclear gene and involved in chloroplast development. Knock-down of transgenic plants displayed albino cotyledons but normal true leaves, while knock-out of the gene in seedlings was lethal. Fluorescent protein analysis showed that ACM1 was specifically localized within chloroplasts. PEP-dependent plastid transcript levels and splicing efficiency of several group II introns were seriously affected in cotyledons in the RNAi line. Furthermore, denaturing gel electrophoresis and Western blot experiments showed that the accumulation of chloroplast ribosomes was probably damaged. Collectively, our results indicate is indispensable in early chloroplast development in cotyledons.
叶绿体的正常发育离不开细胞核和叶绿体基因组之间各种蛋白质的协调作用和信号连接。关于这些过程的许多问题仍未得到解答。在这里，我们报道了一种新型 P 型五肽重复（PPR）因子，命名为 Albino Cotyledon Mutant1（ACM1），它由核基因编码，参与叶绿体的发育。转基因植物的敲低显示出白化的子叶，但正常的真叶，而幼苗中 基因的敲除是致命的。荧光蛋白分析表明，ACM1 特异性定位于叶绿体中。在 RNAi 系的子叶中，PEP 依赖性的质体转录物水平和几个 II 组内含子的剪接效率受到严重影响。此外，变性凝胶电泳和 Western blot 实验表明，叶绿体核糖体的积累可能受到了损害。总之，我们的结果表明，在 子叶的早期叶绿体发育中， 是必不可少的。
