Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Ctra. La Alberca s/n, 30150 Murcia, Spain.
Molecules. 2021 Mar 17;26(6):1689. doi: 10.3390/molecules26061689.
In recent years, it has been demonstrated that the application of elicitors such as methyl-jasmonate (MeJ) and benzothiadiazole (BTH) to wine grapes can increase their phenolic and aromatic compounds if they are treated at the beginning of ripening (veraison). However, the veraison period is short, and it is not always possible to apply the treatments in a few days. Therefore, it would be of great interest to optimize the moment of elicitor application or extend the treatment period. The aim of this paper was to analyze during two consecutive years (2016-2017) the foliar application of MeJ, BTH, and a combination of both, during two different ripening periods of Monastrell grapes (veraison and mid-ripening), and determine the more appropriate moment to increase the concentration of anthocyanins. To carry out this aim, analysis of anthocyanins by HPLC in grapes and wines was mainly performed. The most suitable period for the application of MeJ, BTH, and MeJ + BTH was at mid-ripening, since the grapes showed a greater accumulation of anthocyanins at harvest. However, the MeJ + BTH treatment applied during veraison also obtained similar results, which would allow extending the application period if necessary. However, the increase in the anthocyanin content of grapes was not reflected in all the wines, which may have been due to reinforcement of the skin cell wall as a result of the application of elicitors. Further analysis is needed to improve the maceration process of the Monastrell grapes and the extraction of the anthocyanins that were increased by the treatments applied in the vineyard.
近年来，已经证明如果在葡萄成熟初期（转色期）应用茉莉酸甲酯（MeJ）和噻苯隆（BTH）等诱导剂处理酿酒葡萄，可以增加其酚类和芳香化合物。然而，转色期很短，并不总是可以在几天内进行处理。因此，优化诱导剂应用的时机或延长处理期将是非常有趣的。本文的目的是在连续两年（2016-2017 年）中分析 MeJ、BTH 以及两者组合在两个不同的 Monastrell 葡萄成熟时期（转色期和中期成熟）叶面喷施，以确定增加花色苷浓度的更合适时机。为了实现这一目标，主要通过 HPLC 对葡萄和葡萄酒中的花色苷进行了分析。MeJ、BTH 和 MeJ + BTH 的最适应用期为中期成熟，因为此时葡萄在收获时花色苷积累更多。然而，在转色期应用 MeJ + BTH 处理也能获得类似的结果，如果需要，还可以延长处理期。然而，葡萄花色苷含量的增加并没有反映在所有葡萄酒中，这可能是由于应用诱导剂增强了果皮细胞壁。需要进一步分析以改进 Monastrell 葡萄的浸渍过程和增加处理在葡萄园应用中增加的花色苷的提取。
