一种用于增强人及兔颊上皮细胞培养以用于细胞治疗的3D聚合物支架平台。
A 3D Polymer Scaffold Platform for Enhanced Culture of Human & Rabbit Buccal Epithelial Cells for Cell Therapies.
作者信息
Katoh Shojiro, Rao Srinivas K, Suryaprakash Vaddi, Horiguchi Akio, Kushibiki Toshihiro, Ojima Kenichiro, Shinchi Masayuki, Iwasaki Masaru, Takeda Masayuki, Senthilkumar Rajappa, Rajmohan Mathaiyan, Karthick Ramalingam, Preethy Senthilkumar, Abraham Samuel Jk
机构信息
II Department of Surgery & Center for Advancing Clinical Research (CACR), University of Yamanashi, Faculty of Medicine, 1110, Shimokato, Chuo, Yamanashi 409-3898, Japan.
出版信息
Tokai J Exp Clin Med. 2021 Apr 20;46(1):1-6.
BACKGROUND
Buccal mucosal epithelial cells show promising application for various regenerative medicine approaches. In this study, we examined the feasibility of culturing rabbit and human buccal mucosal epithelial cells in a novel thermoreversible gelation polymer (TGP) scaffold, without feeder layers or other foreign proteins.
METHODS & RESULTS: The results of this 28-day culture, u sing the conventional technique (2D) and TGP (3D) showed that the epithelial cell morphology could be maintained only in the TGP group while cells in the 2D group de-differentiated to fibroblast morphology in both human and rabbit samples. CK3 expression, a marker for epithelial differentiation was higher in 3D-TGP cultured cells than 2D.
CONCLUSION
TGP based cell culture is a prospective methodology to culture buccal mucosal epithelial cells efficiently without using foreign biological components for tissue engineering applications.
背景
颊黏膜上皮细胞在各种再生医学方法中显示出有前景的应用。在本研究中，我们研究了在一种新型热可逆凝胶化聚合物（TGP）支架中培养兔和人颊黏膜上皮细胞的可行性，无需饲养层或其他外源蛋白。
方法与结果
这项为期28天的培养结果，采用传统技术（二维）和TGP（三维）显示，上皮细胞形态仅在TGP组中得以维持，而二维组中的细胞在人和兔样本中均去分化为成纤维细胞形态。CK3表达作为上皮分化的标志物，在三维TGP培养的细胞中高于二维培养的细胞。
结论
基于TGP的细胞培养是一种有前景的方法，可在不使用外源生物成分的情况下高效培养颊黏膜上皮细胞，用于组织工程应用。
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