光子计数相机在用于蛋白质印迹的高灵敏度、生物发光增强检测系统中的应用。蛋白质印迹和DNA杂交的超灵敏检测系统，II。
The application of a photon-counting camera in very sensitive, bioluminescence-enhanced detection systems for protein blotting. Ultrasensitive detection systems for protein blotting and DNA hybridization, II.
作者信息
Hauber R, Miska W, Schleinkofer L, Geiger R
机构信息
Abteilung für Klinische Chemie, Chirurgischen Klinik Innenstadt, Universität München.
出版信息
J Clin Chem Clin Biochem. 1988 Mar;26(3):147-8.
A relatively simple, very sensitive bioluminescence-enhanced detection system for protein blots was described recently. This method utilizes antibodies conjugated with alkaline phosphatase. Alkaline phosphatase releases D-luciferin (Photinus pyralis) from D-luciferin-O-phosphate. Liberated D-luciferin reacts with luciferase, ATP and oxygen with light emission. The light produced is measured with a very sensitive photon counting camera (Argus 100), permitting visualization and localization of the alkaline phosphatase-conjugated antibodies on nitrocellulose sheets. Under non-optimized conditions the limit of detection is at present 5 to 500 fg of protein (rabbit immunoglobulin G), corresponding to 30 to 3 amol. The method is therefore 10(5) times more sensitive than other used at present.
最近描述了一种用于蛋白质印迹的相对简单、非常灵敏的生物发光增强检测系统。该方法利用与碱性磷酸酶偶联的抗体。碱性磷酸酶从D-荧光素-O-磷酸酯中释放出D-荧光素（萤火虫荧光素）。释放出的D-荧光素与荧光素酶、ATP和氧气反应并发光。所产生的光用非常灵敏的光子计数相机（Argus 100）进行测量，从而可以在硝酸纤维素膜上可视化并定位碱性磷酸酶偶联的抗体。在非优化条件下，目前的检测限为5至500 fg蛋白质（兔免疫球蛋白G），相当于30至3 amol。因此，该方法比目前使用的其他方法灵敏10^5倍。
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