Key Laboratory of Soybean Biology in Chinese Education Ministry (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin, China.
Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Chinese Academy of Agricultural Sciences, Beijing, China.
Plant Cell Environ. 2021 Aug;44(8):2777-2792. doi: 10.1111/pce.14066. Epub 2021 May 13.
Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1, on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2-S and RSVG2-R alleles. In RSVG2-S allele carrier "Hefeng25", the overexpression of GmST1 carrying the RSVG2-R allele from the SMV-resistant line "Dongnong93-046" conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2-R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signalling, biosynthesis and catabolism in RSVG2-R and RSVG2-S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV.
大豆花叶病毒（SMV）是全球分布最广泛、破坏性最大的病毒病之一。大豆对 SMV 的定性抗性的遗传结构仍不清楚。本研究通过全基因组关联和连锁分析，鉴定出 Rsvg2 位点是大豆对 SMV 抗性的基础。精细定位结果表明，大豆对 SMV 株系 G2 和 G3 的抗性由位于 13 号染色体上的单个显性基因 GmST1 控制，该基因编码磺基转移酶（SOT）。编码区 506 位的关键变异与编码 SOT 的结构以及 RSVG2-S 和 RSVG2-R 等位基因之间的 SOT 活性水平有关。在携带 RSVG2-S 等位基因的“合丰 25”中，来自抗 SMV 系“东农 93-046”的携带 RSVG2-R 等位基因的 GmST1 的过表达赋予其对 SMV 株系 G2 和 G3 的抗性。与合丰 25 相比，携带 RSVG2-R 等位基因的转基因植株中 SMV 的积累减少。SMV 感染导致 RSVG2-R 和 RSVG2-S 等位基因载体中茉莉酸（JA）信号转导、生物合成和代谢相关基因的茉莉酸积累和表达模式发生分化。GmST1 的这种特征表明了一种新的解释大豆对 SMV 抗性的情景。
