玉米中的序列特异性扩增多态性（SSAP）和序列特征性扩增区域（SCAR）标记

Sequence-Specific Amplified Polymorphism (SSAP) and Sequence Characterized Amplified Region (SCAR) Markers in Zea mays.

作者信息

Roy Neha Samir, Ramekar Rahul Vasudeo, Kim Nam-Soo

机构信息

Department of Agriculture and Life Industry, Kangwon National University, Chuncheon, South Korea.

Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, South Korea.

出版信息

Methods Mol Biol. 2021;2250:207-218. doi: 10.1007/978-1-0716-1134-0_20.

DOI:10.1007/978-1-0716-1134-0_20

PMID:33900607

Abstract

Transposable elements (TEs) are mobile, recurring DNA sequences scattered throughout genome and have a large impact on genome structure and function. Several genetic marker techniques were developed to exploit their ubiquitous nature. Sequence-specific amplified polymorphism (SSAP) is a TE-based genetic marker system that has been used in various purposes such as measuring genetic relatedness between species, deciphering the population structures, molecular tagging for agronomic development in marker-assisted breeding (MAS). In addition to SSAP, sequence characterized amplified region (SCAR) from the SSAP markers provides an added advantage in identifying qualitative traits. Once developed SCAR markers are efficient, fast, and reliable method for genetic evaluations. These methods can be useful especially for the crops which have no genetic sequence information. With improved discriminatory ability they offer access to dynamic and polymorphic regions of genome. These techniques can be useful in breeding programs to improve or develop high yielding crops.

摘要

转座元件（TEs）是散布于整个基因组中的可移动的重复DNA序列，对基因组结构和功能有很大影响。人们开发了几种遗传标记技术来利用它们的普遍存在特性。序列特异性扩增多态性（SSAP）是一种基于转座元件的遗传标记系统，已被用于各种目的，如测量物种间的遗传相关性、解读种群结构、在标记辅助育种（MAS）中用于农艺性状开发的分子标记。除了SSAP，从SSAP标记中获得的序列特征性扩增区域（SCAR）在鉴定质量性状方面具有额外优势。一旦开发出来，SCAR标记是进行遗传评估的高效、快速且可靠的方法。这些方法尤其对没有遗传序列信息的作物有用。凭借提高的鉴别能力，它们能够进入基因组的动态和多态区域。这些技术在育种计划中有助于改良或培育高产作物。