Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Department of Hepatobiliary Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210029, Jiangsu Province, China.
J Exp Clin Cancer Res. 2021 Apr 27;40(1):142. doi: 10.1186/s13046-021-01949-z.
Breast cancer is a heterogeneous disease with a complex microenvironment consisting of tumor cells, immune cells, fibroblasts and vascular cells. These cancer-associated cells shape the tumor microenvironment (TME) and influence the progression of breast cancer and the therapeutic responses in patients. The exact composition of the intra-tumoral cells is mixed as the highly heterogeneous and dynamic nature of the TME. Recent advances in single-cell technologies such as single-cell DNA sequencing (scDNA-seq), single-cell RNA sequencing (scRNA-seq) and mass cytometry have provided new insights into the phenotypic and functional diversity of tumor-infiltrating cells in breast cancer. In this review, we have outlined the recent progress in single-cell characterization of breast tumor ecosystems, and summarized the phenotypic diversity of intra-tumoral cells and their potential prognostic relevance.
乳腺癌是一种具有复杂微环境的异质性疾病，由肿瘤细胞、免疫细胞、成纤维细胞和血管细胞组成。这些与癌症相关的细胞塑造了肿瘤微环境（TME），并影响着乳腺癌的进展和患者的治疗反应。由于 TME 具有高度异质性和动态性，肿瘤内细胞的具体组成是混合的。单细胞技术的最新进展，如单细胞 DNA 测序（scDNA-seq）、单细胞 RNA 测序（scRNA-seq）和质谱流式细胞术，为乳腺癌中浸润肿瘤细胞的表型和功能多样性提供了新的见解。在这篇综述中，我们概述了乳腺癌肿瘤生态系统单细胞特征的最新进展，并总结了肿瘤内细胞的表型多样性及其潜在的预后相关性。
