小鼠 T 辅助细胞分化和可塑性。

Murine T-Helper Cell Differentiation and Plasticity.

机构信息

Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

出版信息

Methods Mol Biol. 2021;2285:65-75. doi: 10.1007/978-1-0716-1311-5_5.

DOI:10.1007/978-1-0716-1311-5_5

PMID:33928543

Abstract

CD4 T helper (T) cells are key mediators of immunity, and according to their effector functions, they can be divided into different subsets, namely, T1, T2, T17, and T22. In order to maintain systemic homeostasis and peripheral tolerance, CD4 T cells are counterbalanced by CD4 T cells with regulatory properties, namely, Foxp3 regulatory T cells (Foxp3T) and T1 cells. Here, we describe how to in vitro differentiate murine naïve CD4 T cells toward helper (T1, T2, T17, and T22) and regulatory (Foxp3T and T1) cells.

摘要

CD4 T 辅助（T）细胞是免疫的关键介质，根据其效应功能，可分为不同亚群，即 T1、T2、T17 和 T22。为了维持全身内环境稳定和外周耐受，CD4 T 细胞被具有调节特性的 CD4 T 细胞（即 Foxp3 调节性 T 细胞（Foxp3T）和 T1 细胞）所平衡。在这里，我们描述了如何在体外将小鼠幼稚 CD4 T 细胞向辅助（T1、T2、T17 和 T22）和调节（Foxp3T 和 T1）细胞分化。