Department of Ophthalmology, Daegu Veterans Health Service Medical Center, 42835, 60 Wolgok-ro, Dalseo-gu, Daegu, Republic of Korea.
Department of Ophthalmology, Pusan National University Yangsan Hospital, 50612, 20 Geumo-ro, Mulgeum-eup, Yangsan, Republic of Korea.
Graefes Arch Clin Exp Ophthalmol. 2021 Oct;259(10):3055-3065. doi: 10.1007/s00417-021-05218-9. Epub 2021 May 7.
To investigate the differences in the eyelid and buccal microbiomes between patients receiving long-term prostaglandin analogs for open-angle glaucoma (PG-OAG) and naïve-OAG patients by using metagenomics.
Eyelid and buccal samples were collected from 30 PG-OAG and 32 naïve-OAG patients. The taxonomic composition of the microbiome was obtained via 16S rRNA gene sequencing, operational taxonomic unit analysis, and diversity analysis. Differential gene expression analysis (DEG) and Bland-Altman (MA) plots were used to determine taxon differences between the microbiomes of PG-OAG and naïve-OAG patients.
The eyelid microbiome showed marginally significant differences, while the alpha-diversity of the buccal microbiome showed significant differences between PG-OAG and naïve-OAG patients. However, the beta-diversity of both eyelid and buccal microbiomes was higher in PG-OAG patients than in naïve-OAG patients. The MA plot showed cluster differences in the eyelid microbiome. DEG analysis of the eyelid microbiome revealed various taxa differences, including enrichment of Azomonas, Pseudomonas, and Granulicatella in PG-OAG patients over naïve-OAG patients, as well as significant depletion of Delftia and Rothia. In the buccal microbiome in PG-OAG patients, taxa such as Rikenella and Stenotrophomonas were significantly enriched.
Our findings suggest that the eyelid microbiome differs between PG-OAG and naïve-OAG patients, raising concerns regarding the eyelid environment in patients receiving these drugs. The overexpressed microbiome in the eyelid area suggests that microbiota may change after the administration of glaucoma medications in OAG.
通过宏基因组学研究，探讨长期使用前列腺素类似物治疗开角型青光眼（PG-OAG）患者与初诊 OAG 患者的眼睑和颊部微生物组之间的差异。
收集 30 例 PG-OAG 和 32 例初诊 OAG 患者的眼睑和颊部样本。通过 16S rRNA 基因测序、操作分类单元分析和多样性分析获得微生物组的分类组成。使用差异基因表达分析（DEG）和 Bland-Altman（MA）图确定 PG-OAG 和初诊 OAG 患者微生物组之间的分类群差异。
PG-OAG 和初诊 OAG 患者的眼睑微生物组显示出边缘显著差异，而颊部微生物组的α多样性显示出显著差异。然而，PG-OAG 患者的眼睑和颊部微生物组的β多样性均高于初诊 OAG 患者。MA 图显示眼睑微生物组存在聚类差异。眼睑微生物组的 DEG 分析显示出各种分类群差异，包括 PG-OAG 患者中 Azomonas、Pseudomonas 和 Granulicatella 的丰度增加，而 Delftia 和 Rothia 的丰度显著减少。在 PG-OAG 患者的颊部微生物组中，Rikenella 和 Stenotrophomonas 等分类群明显富集。
我们的研究结果表明，PG-OAG 和初诊 OAG 患者的眼睑微生物组存在差异，这引发了人们对接受这些药物治疗的患者眼睑环境的关注。眼睑区域过度表达的微生物组表明，在 OAG 患者使用青光眼药物后，微生物群可能会发生变化。
Zotero 插件