α-银环蛇毒素的结构研究。2. 通过改进的接力相干转移核Overhauser增强实验进行的¹H NMR归属

Structural studies of alpha-bungarotoxin. 2. 1H NMR assignments via an improved relayed coherence transfer nuclear overhauser enhancement experiment.

作者信息

Basus V J, Scheek R M

机构信息

Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.

出版信息

Biochemistry. 1988 Apr 19;27(8):2772-5. doi: 10.1021/bi00408a017.

DOI:10.1021/bi00408a017

PMID:3401448

Abstract

Complete sequence-specific assignments of the 1H NMR spectrum of bungarotoxin were reported in the previous paper [Basus, V.J., Billeter, M., Love, R.A., Stroud, R.M., & Kuntz, I.D. (1988) Biochemistry (first paper of three in this issue)]. The assignment was significantly aided by the use of the homonuclear Hartman-Hahn relayed coherence transfer nuclear Overhauser enhancement spectroscopy experiment (HRNOESY) which we present here, as a modification of relayed coherence transfer nuclear Overhauser enhancement spectroscopy (relayed NOESY) [Wagner, G. (1984) J. Magn. Reson. 57, 497]. As shown here, HRNOESY resolves problems of proton resonance overlap especially in extended chain conformations as found in beta-sheets.

摘要

在前一篇论文中报道了银环蛇毒素1H NMR谱的完整序列特异性归属[巴苏斯，V.J.，比勒特，M.，洛夫，R.A.，斯特劳德，R.M.，& 孔茨，I.D.（1988年）《生物化学》（本期三篇论文中的第一篇）]。使用本文介绍的同核哈特曼-哈恩中继相干转移核Overhauser增强光谱实验（HRNOESY）极大地辅助了归属，HRNOESY是中继相干转移核Overhauser增强光谱（中继NOESY）的一种改进[瓦格纳，G.（1984年）《磁共振杂志》57卷，497页]。如本文所示，HRNOESY解决了质子共振重叠的问题，尤其是在β-折叠中发现的延伸链构象中。