失调的 circ_0004913、circ_0008160、circ_0000517 及其作为肝细胞癌疾病监测和预后生物标志物的潜力。
Dysregulated circ_0004913, circ_0008160, circ_0000517, and their potential as biomarkers for disease monitoring and prognosis in hepatocellular carcinoma.
机构信息
Department of Digestive Medical, Affiliated Nanhua Hospital, University of South China, Hengyang, China.
Department of Emergency, Affiliated Nanhua Hospital, University of South China, Hengyang, China.
出版信息
J Clin Lab Anal. 2021 Jun;35(6):e23785. doi: 10.1002/jcla.23785. Epub 2021 May 21.
OBJECTIVE
Circular RNA_0004913 (circ_0004913), circular RNA_0008160 (circ_0008160), and circular RNA_0000517 (circ_0000517) are shown to be dysregulated in HCC tissues and cell lines, and also show potential in regulating hepatocellular carcinoma (HCC) pathogenesis. This current study attempted to find possible associations of circ_0004913, circ_0008160, and circ_0000517 with clinical features and prognosis of HCC patients.
METHODS
A hundred and fifty HCC patients who received hepatectomy were retrospectively reviewed, and their resected specimens including tumor tissues and paired adjacent tissues were obtained, in which the circ_0004913, circ_0008160, and circ_0000517 expressions were detected. Overall survival (OS) data were collected according to the clinical visit records.
RESULTS
Circ_0004913 (p < 0.001) and circ_0008160 (p < 0.001) were downregulated, while circ_0000517 (p < 0.001) was upregulated in tumor tissue compared with paired adjacent tissue. Additionally, tumor circ_0004913 was negatively associated with largest tumor size (p = 0.009) and Barcelona clinic liver cancer (BCLC) stage (p = 0.020), while tumor circ_0008160 and circ_0000517 were not correlated with any clinicopathological features of HCC patients (all p > 0.05). Tumor circ_0004913 high expression was associated with prolonged OS in total HCC patients (p = 0.008) and in subgroup of patients with largest tumor size <5 cm (p = 0.008). Tumor circ_0008160 high expression was correlated with longer OS in patients with BCLC stage B (p = 0.026). Univariate Cox's analysis disclosed that tumor circ_0004913 high expression was correlated with longer OS; while after adjustment by multivariate Cox's analysis, it failed to predict OS independently.
CONCLUSION
Circ_0004913 was downregulated in tumor tissue and may serve as a biomarker for evaluating disease severity and prognosis in HCC patients.
目的
Circ_0004913（circ_0004913）、Circ_0008160（circ_0008160）和 Circ_0000517（circ_0000517）在 HCC 组织和细胞系中表达失调，并且具有调节肝细胞癌（HCC）发病机制的潜力。本研究试图寻找 circ_0004913、circ_0008160 和 circ_0000517 与 HCC 患者临床特征和预后的可能关联。
方法
回顾性分析 150 例接受肝切除术的 HCC 患者，获取其切除标本，包括肿瘤组织和配对的相邻组织，检测 circ_0004913、circ_0008160 和 circ_0000517 的表达。根据临床随访记录收集总生存期（OS）数据。
结果
与配对的相邻组织相比，肿瘤组织中 circ_0004913（p<0.001）和 circ_0008160（p<0.001）下调，而 circ_0000517（p<0.001）上调。此外，肿瘤 circ_0004913 与最大肿瘤大小（p=0.009）和巴塞罗那临床肝癌（BCLC）分期（p=0.020）呈负相关，而肿瘤 circ_0008160 和 circ_0000517 与 HCC 患者的任何临床病理特征均无相关性（均 p>0.05）。肿瘤 circ_0004913 高表达与总 HCC 患者的总生存期延长相关（p=0.008），与肿瘤最大直径<5cm 的亚组患者的总生存期延长相关（p=0.008）。肿瘤 circ_0008160 高表达与 BCLC 分期 B 的患者的 OS 延长相关（p=0.026）。单因素 Cox 分析表明，肿瘤 circ_0004913 高表达与 OS 延长相关；而多因素 Cox 分析调整后，其不能独立预测 OS。
结论
肿瘤组织中 circ_0004913 下调，可能作为评估 HCC 患者疾病严重程度和预后的生物标志物。
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