胎儿酒精综合征的胎儿脑损伤模型：使用 Sholl 分析检测神经元形态状况。

Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.

机构信息

Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.

Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University , Philadelphia, PA, USA.

出版信息

Methods Mol Biol. 2021;2311:195-201. doi: 10.1007/978-1-0716-1437-2_16.

DOI:10.1007/978-1-0716-1437-2_16

PMID:34033088

Abstract

The lack of a convenient in vitro human neuronal model to study alcohol-induced neurodegenerative diseases, such as fetal alcohol syndrome (FAS), prompted us to develop human neuronal culture and in vitro human FAS model by incubating cells with physiologically relevant EtOH concentration (50 mM). Here, we describe the detailed method of isolation of human neuronal culture, and ability to analyze neurites extension using Sholl assay. We utilized highly efficient transfection method of neuronal cells to study morphology of neurons with or without EtOH treatment.

摘要

缺乏一种方便的体外人类神经元模型来研究酒精引起的神经退行性疾病，如胎儿酒精综合征 (FAS)，这促使我们通过在细胞中孵育生理相关浓度的乙醇（50mM）来开发人类神经元培养和体外人类 FAS 模型。在这里，我们描述了分离人类神经元培养物的详细方法，以及使用 Sholl 分析来分析神经突延伸的能力。我们利用神经元细胞的高效转染方法来研究有无乙醇处理的神经元的形态。