三维打印聚（L-乳酸）和羟基磷灰石复合材料修复兔临界骨缺损。

Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits.

机构信息

Assistant Professor. Universidade Estadual Paulista "Júlio de Mesquita Filho" - Faculty of Agrarian and Veterinary Sciences - Department of Clinical and Veterinary Surgery - Jaboticabal (SP), Brazil.

Assistant Professor. Centro Universitário de Maringá - Department of Veterinary Anatomy - Maringá (PR), Brazil.

出版信息

Acta Cir Bras. 2021 May 21;36(4):e360404. doi: 10.1590/ACB360404. eCollection 2021.

DOI:10.1590/ACB360404

PMID:34037081

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8148815/

Abstract

PURPOSE

To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits.

METHODS

A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days).

RESULTS

The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation.

CONCLUSIONS

The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group.

摘要

目的

使用 3D 打印聚（L-乳酸）酸（PLLA）和羟基磷灰石（HA）复合材料作为骨替代物，重建兔桡骨的临界骨缺损。

方法

在 60 只新西兰白兔桡骨干进行 1.5cm 截肢术。根据骨缺损的手术治疗将兔子分为三组（I 组-对照，II 组-骨移植，III 组-3D PLLA）。每组分为四个亚组，具有不同的影像学和组织病理学评估时间（T1-15 天，T2-30 天，T3-60 天，T4-90 天）。

结果

植入组的临床跛行（p=0.02）、肿胀（p=0.007）、疼痛（p=0.04）和手术部位并发症（p=0.03）更多。组织学上，该组显示出更大的充血（p=0.04）、出血（p=0.04）和炎症。尽管影像学上，移植物组（II）的骨愈合更有效，形成更多的骨痂和骨桥，但在天（p=0.54）和治疗（p=0.17）之间，成骨作用在微观上相似。