miR-26a 调控骨质疏松症患者血清 IGF-1 的表达及其对小鼠软骨细胞增殖和凋亡的影响。

MiR-26a regulates the expression of serum IGF-1 in patients with osteoporosis and its effect on proliferation and apoptosis of mouse chondrocytes.

机构信息

Orthopedic Department, Rizhao People's Hospital, P.R. China.

Dermatology Department, Rizhao People's Hospital, P.R. China.

出版信息

J Musculoskelet Neuronal Interact. 2021 Jun 1;21(2):298-307.

OBJECTIVES

To examine the effects of the regulation on IGF-1 by miR-26a on the serum of patients with osteoporosis (OP) and apoptosis and proliferation of chondrocytes of mice with OP.

METHODS

Totally 47 patients with OP treated in our hospital between July 2018 and November 2019 were selected as the research group, and 42 healthy individuals in physical examination over this period were selected as the control group. Serum was sampled from each participant in both groups, and miR-26a in the sampled serum was quantified and compared. In addition, chondrocytes were sampled from mice with OP. The changes of proliferation and apoptosis of the chondrocytes were analyzed via MTT and flow cytometry, and the levels of Caspase3, Caspase9, Bax, and Bcl-2 were quantified by western blot (WB) assay.

RESULTS

MiR-26a was expressed highly in the serum of patients with OP and chondrocytes of mice with OP, while IGF-1 was lowly expressed in them. According to the dual-luciferase reporter assay, there was a targeting correlation between miR-26a and IGF-1, and suppressing miR-26a significantly up-regulated the expression and protein level of IGF-1.

CONCLUSIONS

MiR-26a can serve as a biological marker for the diagnosis of OP, and it can suppress the proliferation of chondrocytes and promote their apoptosis by regulating IGF-1.

目的

研究 miR-26a 对 IGF-1 的调控作用对骨质疏松症（OP）患者血清及 OP 模型小鼠软骨细胞凋亡和增殖的影响。

方法

选取我院 2018 年 7 月至 2019 年 11 月收治的 47 例 OP 患者作为研究组，选取同期体检的 42 例健康人作为对照组。采集两组研究对象的血清，对采集的血清进行 miR-26a 定量检测并比较。另外，从 OP 模型小鼠中采集软骨细胞，通过 MTT 及流式细胞术分析软骨细胞的增殖和凋亡变化，通过 Western blot（WB）assay 检测 Caspase3、Caspase9、Bax 和 Bcl-2 水平。